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  • Bacteria  (2)
  • Biosurfactant  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Production of extracellular emulsifying agent byPseudomonas aeruginosa UG1 (1990)
    Springer
    Journal of industrial microbiology and biotechnology 5 (1990), S. 25-31 
    Details
    ISSN: 1476-5535
    Keywords: Oil ; Emulsifier ; Bacteria ; Pseudomonas aeruginosa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Twenty-three bacterial strains were isolated from oil-contaminated soil samples. Of these, 20 displayed some ability to effect oil dispersion and they were screened quantitatively for the ability to emulsify 0.5% (v/v) reference oil. One strain, identified asPseudomonas aeruginosa UG1, produced extracellular material that emulsified reference oil, hexadecane and 2-methylnaphthalene at concentrations as high as 6% (v/v) in nutrient broth. Emulsification activity increased during a 10 day incubation period at 30°C. The activity was not influenced by pH over the range 5 to 9. The emulsifying agent was precipitated by cold ethanol. The highest emulsifying activity was detected in the extracellular fraction precipitated between 30 and 50% (v/v) ethanol. A linear relationship was observed between emulsifier concentration (mg/ml) and emulsifying activity. Genetic analysis showed that thePseudomonas aeruginosa UG1 strain did not carry extrachromosomal plasmids, suggesting that the gene(s) coding for emulsifying activity was carried on the chromosome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01569603
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  • 2
    Electronic Resource
    Electronic Resource
    Germanium accumulation by bacteria (1989)
    Springer
    Archives of microbiology 152 (1989), S. 533-538 
    Details
    ISSN: 1432-072X
    Keywords: Accumulation ; Bacillus ; Bacteria ; Catechol ; Germanium ; Metal ; Pseudomonas
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Germanium accumulation was investigated in 23 bacterial strains. Bacillus strains accumulated the most Ge. Increasing the pH of the incubation medium from 7 to 8.5, as well as substituting catechol for glucose resulted in increased Ge accumulation. The apparent K s and V max of Ge accumulation in Bacillus cereus NRC 3045 were found to be 4.0 g/l and 2.2 mg/g dry wt/h, respectively. When cells from three different Bacillus strains were incubated in the presence of 2,4-dinitrophenol or toluene, Ge accumulation was completely inhibited. At 6° C, two out of three Bacillus strains showed a large decrease in Ge accumulation. In addition, non-viable Bacillus cells killed by UV irradiation did not accumulate Ge. These results strongly suggest that Ge accumulation by some Bacillus strains may be an energy-dependent process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425482
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  • 3
    Electronic Resource
    Electronic Resource
    Effect of addition ofPseudomonas aeruginosa UG2 inocula or biosurfactants on biodegradation of selected hydrocarbons in soil (1992)
    Springer
    Journal of industrial microbiology and biotechnology 10 (1992), S. 87-93 
    Details
    ISSN: 1476-5535
    Keywords: Availability ; Biosurfactant ; Biodegradation ; Bioemulsifier ; Hydrocarbon ; Persistence ; Pseudomonas aeruginosa ; Solubility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A laboratory study was undertaken to assess the effect of adding eitherPseudomonas aeruginosa UG2 cells or the biosurfactants produced by this m microorganism on the biodegradation of a hydrocarbon mixture in soil at 20°C over a 2-month incubation period. The addition of 100 μg of UG2 biosurfactants per g soil significantly enhanced the degradation of tetradecane, hexadecene and pristane but not 2-methylnaphthalene, the most water-soluble of the hydrocarbons. Addition of UG2 cells at densities of 106, 107, and 108 per g soil did not have a significant effect on biodegradation of the hydrocarbon mixture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01583840
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  • 4
    Electronic Resource
    Electronic Resource
    Evaluation of microbial surfactants for recovery of hydrophobic pollutants from soil (1993)
    Springer
    Journal of industrial microbiology and biotechnology 11 (1993), S. 163-170 
    Details
    ISSN: 1476-5535
    Keywords: Acinetobacter calcoaceticus ; Bioemulsifier ; Biosurfactant ; Hydrophobic ; Hydrocarbon ; Pseudomonas aeruginosa ; Partitioning ; Recovery ; Remediation ; Soil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Several microbially produced biosurfactants were evaluated for their ability to remove hydrophobic compounds from soil. The biosurfactants produced byPseudomonas aeruginosa UG2 andAcinetobacter calcoaceticus RAG-1 displayed the best results, with recovery of [14C]hexachlorobiphenyl from soil slurries of 48.0 and 41.9%, respectively.P. aeruginosa UG2 produced higher levels of extracellular biosurfactants than four otherP. aeruginosa strains.P. aeruginosa UG2 culture filtrate containing biosurfactants enhanced the recovery of several other individual hydrocarbons and polychlorinated biphenyl compounds, as well as several hydrocarbons in a mixture, from soil. The results, suggest that biosurfactants produced byP. aeruginosa UG2 have the potential for remediation of hydrophobic pollutants in soil environments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01583718
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