ISSN:
1572-9699
Keywords:
Bacillus thuringiensis PG-14
;
mosquitocidal activity
;
65-kDa protein
;
affinity purification
;
25-kDa protein
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract By using antibody-mediated affinity chromatography, a highly mosquito larvicidal but nonhemolytic fraction was obtained from alkali-solubilized, silkworm (Bombyx mori) larval gut juice-treated parasporal inclusions of Bacillus thuringiensis strain PG-14 (serotype 8a : 8b). This fraction contained a 65-kDa protein only but not a 25-kDa protein, the main component in the flow through fraction unbound to the affinity column. The 25-kDa protein purified from the unbound fraction by CM-cellulose chromatography demonstrated a high hemolytic activity against sheep red blood cells but very low mosquito larvicidal activity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00443584
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