ISSN:
1612-1112
Keywords:
Capillary Zone Electrophoresis
;
Bacillus subtilis
;
Tryptophanyl-tRNA synthetase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary The application of capillary zone electrophoresis to the study of interactions betweenBacillus subtilis tryptophanyl-tRNA synthetase (TrpRS) and tRNATrp is described. Significant changes in peak shape of tRNATrp incubated with TrpRS indicated the occurrence of interactions between TrpRS and tRNATrp in pH 8.0 Tris-HCl buffer containing 0.1 mmol L−1 EDTA and 1 mmol L−1−5 mmol L−1 mgCl2. Addition of Mg2+ decreased the electrophoretic mobility of tRNATrp, which illustrated that conformation of tRNATrp depended on Mg2+. The dissociation constant of the TrpRS-tRNATrp complex was estimated to be 0.63 μmol L−1 at 25°C in buffer solution.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02467682
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