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  • Bacillus cereus  (1)
  • FITC-labelling  (1)
  • 1
    Digitale Medien
    Digitale Medien
    Binding and activity ofBacillus thuringiensis delta-endotoxin to invertebrate cells (1986)
    Springer
    Archives of microbiology 146 (1986), S. 7-11 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Bacillus thuringiensis ; Delta-endotoxin ; Mode of action ; Binding specificity ; FITC-labelling ; Antibodies
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Fluorescein isothiocyanate was used as a label to detect delta-endotoxin ofBacillus thuringiensis subsp.thuringiensis andisraelensis in binding studies with different in vitro cell systems. Protoxin of the subspeciesthuringiensis could be labelled directly whereas the activated toxin had to be traced indirectly with labelled antibodies. Both protoxin and activated toxin bound to primary midgut cell cultures ofPieris brassicae larvae as well as to cells of an established culture ofDrosophila melanogaster. No binding with either toxin form could be observed with hemocytes ofP. brassicae. Biological activity as shown by the trypan blue viability assay was obtained only with the activated toxin against the midgut cells. Toxin of the subspeciesisraelensis reacted very unspecifically. Binding followed by rapid destruction was obtained with all the tested cultures.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00690150
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Bacterial mesosomes: Method dependent artifacts (1981)
    Springer
    Archives of microbiology 130 (1981), S. 19-22 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Bacillus cereus ; Mesosomes ; Chemical fixation ; Cryofixation ; Freeze-substitution ; Freeze-fracturing
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The occurrence of mesosomes was investigated during septum formation of vegetative and sporulating cells of Bacillus cereus. It has been demonstrated that bacterial mesosomes which are considered by numerous microbiologists as an integrated constituent of Gram positive bacteria, are in reality artifacts arising during the preparation for electron microscopy. The conventional fixation methods allowed enough time for the cytoplasmic membrane to react to the changed conditions and to form the typical pocket-like membrane invaginations. With cryofixation followed by freeze-substitution it was shown in ultrathin sections that mesosomes do not occur. The extremely rapid freezing and the substitution of the ice by an organic solvent containing the fixative prevented the formation of membraneous artifacts.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00527066
    Standort Signatur Erwartet Verfügbarkeit
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    Artikel (Nationallizenzen)
    Volltext
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