ISSN:
1476-5535
Keywords:
gramicidin S
;
Bacillus brevis
;
spore germination
;
calcium
;
germination outgrowth
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract Gramicidin S (GS) inhibition of germination outgrowth ofBacillus brevis spores was reversed completely by a short pretreatment with sodium dodecyl sulfate, moderately by ethanol or by incubation at pH 10 but not by incubation at pH 4. Of five metal ions tested (Na+, Mg2+, Fe2+, Cu2+, Ca2+), only Ca2+ reversed GS inhibition. When Ca2+ (but not the other four metal ions) was added to the growth medium, there was a considerable portion of the biosynthesized GS found in the extracellular fluid. These findings are interpreted in terms of the binding of GS to the external layers of theB. brevis spore.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01570150
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