ISSN:
1432-072X
Keywords:
Accumulation
;
Bacillus
;
Bacteria
;
Catechol
;
Germanium
;
Metal
;
Pseudomonas
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Germanium accumulation was investigated in 23 bacterial strains. Bacillus strains accumulated the most Ge. Increasing the pH of the incubation medium from 7 to 8.5, as well as substituting catechol for glucose resulted in increased Ge accumulation. The apparent K s and V max of Ge accumulation in Bacillus cereus NRC 3045 were found to be 4.0 g/l and 2.2 mg/g dry wt/h, respectively. When cells from three different Bacillus strains were incubated in the presence of 2,4-dinitrophenol or toluene, Ge accumulation was completely inhibited. At 6° C, two out of three Bacillus strains showed a large decrease in Ge accumulation. In addition, non-viable Bacillus cells killed by UV irradiation did not accumulate Ge. These results strongly suggest that Ge accumulation by some Bacillus strains may be an energy-dependent process.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00425482
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