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  • 1
    ISSN: 1573-4935
    Keywords: microcarrier ; culture ; BRIN-BD11 ; insulin secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In view of the advantages of the bulk production of clonal pancreaticbeta cells, an investigation was made of the growth and insulin secretoryfunctions of an electrofusion-derived cell line (BRIN-BD11) immobilizedon a solid microcarrier, cytodex-1 or a macroporous microcarrier,cultispher-G. For comparison, similar tests were performed usingBRIN-BD11 cells present in single cell suspensions or allowed toform pseudoislets. Similar growth profiles were recorded for eachmicrocarrier with densities of 4.4×105±0.3 cells/ml and4.2×105±0.2 cells/ml achieved using cytodex-1 andcultispher-G, respectively. Cell viability began to decline on day 5 ofculture. Insulin concentration in the culture medium reached a peak of26±2.0 ng/ml and 24±2.2 ng/ml for cells grown oncytodex-1 and cultispher-G, respectively. Cells grown on both types ofmicrocarrier showed a significant 1.5–1.8-fold acuteinsulin-secretory response to 16.7 mmol/l glucose. L-alanine (10 mmol/l) andL-arginine (10 mmol/l) also induced significant 3–4 fold increasesof insulin release. BRIN-BD11 cells immobilized on cytodex-1 or cultispher-Gout-performed single cell suspensions and pseudoislets in terms ofinsulin-secretory responses to glucose and amino acids. A 1.3-fold,2.2-fold and 1.7-fold stimulation of insulin secretion was observed forglucose, L-alanine and L-arginine respectively in single cellsuspensions. Corresponding increases for pseudoislets were1.6–1.8-fold for L-alanine and L-arginine, with no significantresponse to glucose alone. These data indicate the utility ofmicro-carriers for the production of functioning clonal beta cells.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part B: Polymer Physics 24 (1986), S. 2631-2642 
    ISSN: 0887-6266
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: PVA films (30-45 μm thickness) were prepared by casting. dc electrical measurements were done over the temperature range 293-353 K. Two different conduction mechanisms are suggested. The first one is ohmic and extends to 303 K. Above this temperature a space-charge-limited conduction mechanism, with protons as charge carriers, predominates. Dependences of the voltage-reversion current temperature and field were investigated and were attributed to a clean-up effect of charge carriers. The variance in the activation energy, calculated from the conductivity curves and from the mobility curves, led to a discussion of the origin of the charge carriers. No change in conductivity was observed for the PVA films irradiated with UV (of wavelength 365 nm) at 303 K, i.e., PVA is a photostable material under these conditions.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 55 (1995), S. 415-420 
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Poly(vinyl alcohol) (PVA) was found to form complexes with PbCl2, Pb(NO3)2, and Pb(CH3COO)2. Different complex compositions were prepared by casting technique. Microdomains were observed in the case of the PbCl2-PVA system. Complexes of PbCl2-PVA system are somicrystalline over the entire composition range. PbCl2-PVA complexes are stable to temperatures greater than 350°C in comparison to 225°C of pure PVA. The general features of the microdomains depends upon the temperature and the salt concentration. The hardness properties of the binary mixture were found to change in a nonlinear mode exhibiting a maximum value with the increase of the PbCl2 content and in a linear mode for the other salts. © 1995 John Wiley & Sons, Inc.
    Additional Material: 4 Ill.
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 59 (1996), S. 655-662 
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The ultraviolet spectra of pure and lead salt-poly(vinyl alcohol) (PVA) composite films were studied at room temperature. Blending of PVA with lead acetate and lead nitrate cause a considerable change in the UV spectrum, indicating electronic structure modifications. The complex permittivity (ε*) and the complex electric modulus (M*) of the pure PVA and the PVA-based composite films were investigated between 300 and 400 K in the 100 Hz-100 kHz frequency range. The frequency dependence of the permittivity is influenced by the space-charge polarization. The interfacial polarization is manifested at high temperature and becomes important only above the PVA glass transition temperature and below 1 kHz. Dielectric properties of PVA-lead nitrate composite revealed that the salt is complexed with PVA through hydrogen bonding. © 1996 John Wiley & Sons, Inc.
    Additional Material: 5 Ill.
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  • 5
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Polymer International 39 (1996), S. 105-111 
    ISSN: 0959-8103
    Keywords: poly(acrylonitrile-butadiene-styrene) ; rhodamine doping ; TSDC spectra ; thermally stimulated depolarization ; characterization ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: IR and UV-absorption spectra, and the thermally stimulated currents of pure and Rhodamine-6G-doped poly(acrylonitrile-butadiene-styrene) (Rhdoped ABS) films were investigated. Structural characteristics could be specified from these techniques. Both IR and UV-absorption studies revealed a modification of the structure of ABS on blending with Rhodamine 6G: Rh molecules are partially dispersed in the ABS matrix and partially attached as side groups to the ABS backbone. Thermally stimulated depolarization current (TSDC) studies confirmed this result. The results revealed that incorporation of Rh 6G in ABS locks the dipole in the ABS matrix after electric poling. The TSDC spectra have been found, depending on the polarization temperature, to be characterized by three peaks. The phenomenon of the existence of these current maxima is discussed and analysed in terms of dipolar and ionic relaxations.
    Additional Material: 4 Ill.
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  • 6
    Electronic Resource
    Electronic Resource
    New York : Wiley-Blackwell
    Die Makromolekulare Chemie 132 (1970), S. 1-5 
    ISSN: 0025-116X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: Die Polymerisation von N-Vinylcarbazol mit HgCl2 in Chlorbenzol ist von cokatalytischen Verunreinigungen abhängig, welche im Monomeren enthalten sind. Diese Verunreinigungen konnten teilweise durch Kristallisation und Zonenschmelzen entfernt werden. Hierdurch wurde die Polymerisation erheblich verlangsamt, konnte aber nicht vollständig unterdrückt werden. Die restliche Reaktivität stammt von cokatalytischen Verunreinigungen, welche durch unsere Methoden nicht entfernt werden konnten.
    Notes: The polymerisation of N-vinylcarbazole by mercuric chloride in chlorobenzene depends on the presence of co-catalytic impurities contained in the monomer. These impurities can be partly removed by crystallisation and zone-refining of the monomer, and these treatments reduce the polymerisation rate considerably, but not to zero; the residual reactivity is due to a co-catalyst which was not removed by our methods.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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