ISSN:
0006-3592
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Resting cell suspensions of Sepedonium ampullosporum have been used successfully in the pilot plant, transformation of 9β 10α-pregna-4, 6-dien-3, 20-dione to its 16α-hydroxy derivative. The resting cell enzyme system acted as a stable respiratory unit up to 150 hr after resuspension in water. Semicontinuous addition of substrate to the same cell suspension reduced overall conversion lime by 67%.Aeration and agitation were important factors affecting conversion rates. The hydroxylating system had a critical oxygen concentration above 90% saturation of air in water. Hydroxylase activity was inhibited by cyanide an d totally inhibited by the respiratory inhibitor antimycin A at concentrations much less that that concentration required to block normal respiration.
Additional Material:
1 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bit.260140512
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