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  • 1
    Electronic Resource
    Electronic Resource
    Two-dimensional versus three-dimensional culture systems: Effects on growth and productivity of BHK cells (1996)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 52 (1996), S. 429-432 
    Details
    ISSN: 0006-3592
    Keywords: BHK ; aggregates ; porous microcarriers ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The influence of surface growth (two-dimensional microcarriers) and three-dimensional growth (aggregates and macroporous supports) in agitated, suspended batch culture systems upon growth and productivity of BHK was compared. Cultures using three porous microcarriers (CultiSpher G, Cellsnow EX, and Cytocell), one nonporous microcarrier (Cytodex 3) and natural aggregates were performed in stirred tanks using two different agitation rates (60 and 100 RPM). With the exception of Cytocell, cell growth, viability, and productivity were similar when three-dimensional structures (porous microcarriers and aggregates) were used. Nonporous microcarriers only compared well at 60 RPM as growth ceased under overagitation. These results suggest that cultures less susceptible to fluid shear are advantageous for scale-up. © 1996 John Wiley & Sons, Inc.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
  • 2
    Electronic Resource
    Electronic Resource
    Coupled thermo-mechanical analysis of metal-forming processes through a combined finite element-boundary element approach (1998)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 42 (1998), S. 631-645 
    Details
    ISSN: 0029-5981
    Keywords: metal forming ; thermo-mechanical ; finite elements ; boundary elements ; Engineering ; Numerical Methods and Modeling
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: This paper presents a new approach for obtaining the distribution of temperature in the dies during thermo-mechanical numerical analysis of metal forming problems. The proposed approach is based on a solution resulting from the combination of the finite element method with the boundary element method.The finite element method is used to perform the numerical modelling of the thermo-mechanical deformation of the workpiece, taking into account the geometrical and material non-linearities as well as the influence of the temperature distribution on the mechanical behaviour of the material. The boundary element method is applied for computing the distribution of temperatures in the dies. The combination of the two numerical methods is made using the finite element solution of the heat flow exchanged across the die-workpiece interface to define the boundary conditions to be applied on the thermal analysis of the dies. A numerical example of compression under plane-strain conditions is included to show the applicability of the proposed approach. © 1998 John Wiley & Sons, Ltd.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
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