ISSN:
1573-0778
Keywords:
anti-idiotype
;
B-chronic lymphocytic leukaemia
;
HAT sensitive mutant
;
human hybridoma
;
lymphoblastoid cell line
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract The potential of a new HAT-sensitive human lymphoblastoid cell line TK6 TGr.P1. as a fusion partner was assessed, by comparison with the established human parental cell line UC729.6. Both of these cell lines were fused with the peripheral blood mononuclear cells of a patient with B-chronic lymphocytic leukaemia. The hybridomas generated in these fusion experiments were analysed by the fluorescence activated cell sorter and karyotyping. An anti-idiotype ELISA assay detected the presence of the patient's characteristic idiotype bearing immunoglobulin in the supernatant of a number of the hybridoma cell lines generated in both fusions.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00365082
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