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  • Azospirillum lipoferum  (1)
  • mutants  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Identification of a promoter dependent on NifA and σ54 upstream ofnifH inAzospirillum lipoferum (1991)
    Springer
    Molecular genetics and genomics 227 (1991), S. 86-90 
    Details
    ISSN: 1617-4623
    Keywords: Azospirillum lipoferum ; Nitrogen fixation ; nifH promoter ; NifA ; σ54
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Southern hybridization experiments strongly indicate that the regulatory region of theAzospirillum lipoferum nifH gene is located on a cloned 1.1 kbBamHI-XhoI restriction fragment. By cloning this fragment into a promoter-probe plasmid inEscherichia coli, a promoter was identified oriented towards thenifH gene. Using a set of several bacterial strains and plasmids, both NifA and the alternative σ factor, σ54, fromKlebsiella pneumoniae were shown to be required for the induction of the assumednifH promoter in this particular heterologous system. However, NtrC fromK. pneumoniae did not stimulate this promoter. No other promoter activity was detected in the direction opposite to the identified promoter, indicating that the transcription of the adjacentnifJ gene cannot be initiated from the 1.1 kbBamHI-XhoI fragment. Thus, the genesnifH andnifJ inA. lipoferum cannot be oriented divergently, in contrast to the situation in several other nitrogen-fixing bacteria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00260711
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  • 2
    Electronic Resource
    Electronic Resource
    Identification of a regulatory nifA type gene and physical mapping of cloned new nif regions of Azospirillum brasilense (1989)
    Springer
    Molecular genetics and genomics 219 (1989), S. 235-240 
    Details
    ISSN: 1617-4623
    Keywords: Azospirillum ; Nif− ; mutants ; nifA ; Cloning ; Complementation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Three new Tn5-mutagenized nif genes of Azospirillum brasilense were characterized. The sizes of the restriction fragments and the restriction maps of the cloned nif DNA regions showed that these nif genes are distinct from those reported earlier, e.g. nifHDK, nifE, nifUS, fixABC. The Nif27 mutant was identified as a nifA type regulatory gene of A. brasilense (a) by genetic complementation with nifA of Klebsiella pneumoniae, (b) by the absence of nitrogenase iron protein in western protein blots and (c) by its inability to activate expression of a nijH-lacZ fusion. The growth characteristics of the three mutants showed that none of them is defective in general nitrogen regulatory (ntr) genes. Also, no homology was detected between the three nif DNA regions of the mutants, cloned in pMS188, pMS189 and pMS197, and the K. pneumoniae nif, gInA or ntr genes. In addition, the fixABC genes of Bradyrhizobium japonicum did not show any hybridization with the cloned Azospirillum genes. Unlike the situation in enteric bacteria, the nif genes in A. brasilense are scattered and span a region of about 65 kb.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00261182
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    Paper (German National Licenses)
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