Publication Date:
1990-03-23
Description:
RNA and DNA expression vectors containing genes for chloramphenicol acetyltransferase, luciferase, and beta-galactosidase were separately injected into mouse skeletal muscle in vivo. Protein expression was readily detected in all cases, and no special delivery system was required for these effects. The extent of expression from both the RNA and DNA constructs was comparable to that obtained from fibroblasts transfected in vitro under optimal conditions. In situ cytochemical staining for beta-galactosidase activity was localized to muscle cells following injection of the beta-galactosidase DNA vector. After injection of the DNA luciferase expression vector, luciferase activity was present in the muscle for at least 2 months.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wolff, J A -- Malone, R W -- Williams, P -- Chong, W -- Acsadi, G -- Jani, A -- Felgner, P L -- HD00669-05/HD/NICHD NIH HHS/ -- HD03352/HD/NICHD NIH HHS/ -- New York, N.Y. -- Science. 1990 Mar 23;247(4949 Pt 1):1465-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pediatrics, Waisman Center, University of Wisconsin, Madison 53706.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1690918" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Avian Sarcoma Viruses/genetics
;
Beetles/genetics
;
Chloramphenicol O-Acetyltransferase/biosynthesis/genetics
;
DNA/genetics
;
Escherichia coli/genetics
;
*Gene Expression
;
Genetic Vectors
;
Histocytochemistry
;
Luciferases/biosynthesis/genetics
;
Mice
;
Muscles/*enzymology
;
RNA/genetics
;
*Transfection
;
beta-Galactosidase/biosynthesis/genetics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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