Publication Date:
2015-05-09
Description:
The present palette of opsin-based optogenetic tools lacks a light-gated potassium (K(+)) channel desirable for silencing of excitable cells. Here, we describe the construction of a blue-light-induced K(+) channel 1 (BLINK1) engineered by fusing the plant LOV2-Jalpha photosensory module to the small viral K(+) channel Kcv. BLINK1 exhibits biophysical features of Kcv, including K(+) selectivity and high single-channel conductance but reversibly photoactivates in blue light. Opening of BLINK1 channels hyperpolarizes the cell to the K(+) equilibrium potential. Ectopic expression of BLINK1 reversibly inhibits the escape response in light-exposed zebrafish larvae. BLINK1 therefore provides a single-component optogenetic tool that can establish prolonged, physiological hyperpolarization of cells at low light intensities.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Cosentino, Cristian -- Alberio, Laura -- Gazzarrini, Sabrina -- Aquila, Marco -- Romano, Edoardo -- Cermenati, Solei -- Zuccolini, Paolo -- Petersen, Jan -- Beltrame, Monica -- Van Etten, James L -- Christie, John M -- Thiel, Gerhard -- Moroni, Anna -- BB/J016047/1/Biotechnology and Biological Sciences Research Council/United Kingdom -- BB/M002128/Biotechnology and Biological Sciences Research Council/United Kingdom -- New York, N.Y. -- Science. 2015 May 8;348(6235):707-10. doi: 10.1126/science.aaa2787. Epub 2015 May 7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biosciences, University of Milano, Italy. ; Institute of Molecular, Cell and Systems Biology, University of Glasgow, UK. ; Department of Plant Pathology and Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE 68583-0900, USA. ; Membrane Biophysics, Technical University of Darmstadt, Darmstadt, Germany. ; Department of Biosciences, University of Milano, Italy. anna.moroni@unimi.it.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/25954011" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Avena/metabolism
;
Biophysical Processes
;
HEK293 Cells
;
Humans
;
Larva
;
Light
;
*Optogenetics
;
Phototropins/chemistry/genetics
;
Potassium Channels/chemistry/genetics
;
Protein Conformation/radiation effects
;
Protein Engineering
;
Recombinant Fusion Proteins/chemistry/genetics/*radiation effects
;
Viral Proteins/chemistry/genetics
;
Zebrafish
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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