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  • Automation  (1)
  • Contractile system  (1)
  • DNA, Viral/genetics  (1)
  • Fluoresceins  (1)
  • 1
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    A system for rapid DNA sequencing with fluorescent chain-terminating dideoxynucleotides (1987)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1987-10-16
    Description: A DNA sequencing system based on the use of a novel set of four chain-terminating dideoxynucleotides, each carrying a different chemically tuned succinylfluorescein dye distinguished by its fluorescent emission is described. Avian myeloblastosis virus reverse transcriptase is used in a modified dideoxy DNA sequencing protocol to produce a complete set of fluorescence-tagged fragments in one reaction mixture. These DNA fragments are resolved by polyacrylamide gel electrophoresis in one sequencing lane and are identified by a fluorescence detection system specifically matched to the emission characteristics of this dye set. A scanning system allows multiple samples to be run simultaneously and computer-based automatic base sequence identifications to be made. The sequence analysis of M13 phage DNA made with this system is described.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Prober, J M -- Trainor, G L -- Dam, R J -- Hobbs, F W -- Robertson, C W -- Zagursky, R J -- Cocuzza, A J -- Jensen, M A -- Baumeister, K -- New York, N.Y. -- Science. 1987 Oct 16;238(4825):336-41.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Engineering Physics Laboratory, E.I. du Pont de Nemours & Company (Inc.), Wilmington, DE 19898.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2443975" target="_blank"〉PubMed〈/a〉
    Keywords: Automation ; Avian Myeloblastosis Virus/enzymology ; Bacteriophages/genetics ; *Base Sequence ; DNA/*genetics ; DNA, Viral/genetics ; *Deoxyribonucleotides/adverse effects/therapeutic use ; Electrophoresis, Polyacrylamide Gel ; Fluoresceins ; *Fluorescent Dyes ; RNA-Directed DNA Polymerase/metabolism ; Spectrometry, Fluorescence ; Succinates
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 2
    Electronic Resource
    Electronic Resource
    Influence of inorganic phosphate and energy state on force in skinned cardiac muscle from freshwater turtle and rainbow trout (1999)
    Springer
    Journal of comparative physiology 169 (1999), S. 439-444 
    Details
    ISSN: 1432-136X
    Keywords: Key words Ca2+ sensitivity ; Contractile system ; Hypoxia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Inorganic phosphate, which increases in the hypoxic cardiac cell, depresses force development. The cardiac muscle of freshwater turtle maintains a remarkably high contractility during hypoxia; this may involve a low sensitivity to phosphate. Therefore, freshwater turtle and rainbow trout were compared with regard to Ca2+-activated force in skinned atrial trabeculae in a bath containing 3 mM ATP buffered by 15 mM creatine phosphate in the presence of creatine kinase. For turtle, an increase in phosphate from 0 mM to either 6 mM or 12 mM reduced maximal force by 50% and 80% respectively, whereas the Ca2+ activity eliciting half maximal force (Ca0.5) was increased by 70% in 6 mM and could not be reliably recorded in 12 mM. For trout, the effects of phosphate were less pronounced. An increase from 0 mM to 12 mM did not affect maximal force significantly, but elevated Ca0.5 by 70%. Hypoxia increases ADP as creatine phosphate is shifted to creatine, therefore, creatine phosphate was changed from 15 mM to 3 mM and creatine from 0 mM to 12 mM. After these changes, the elevation of phosphate from 0 mM to 12 mM had no significant effects for either turtle or trout. In conclusion, the high performance of turtle cardiac muscle during hypoxia does not involve a low sensitivity of the contractile system to phosphate. In addition, the effect of increased phosphate seems to be offset by a concomitant increase in ADP.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003600050240
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