ISSN:
1612-1112
Keywords:
Liquid chromatography
;
Photochemical reaction detection
;
Chemical post-column reduction
;
Fluorimetric detection
;
Air segmented flow systems
;
Non-aqueous reversed-phase chromatography
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary A new photochemical reaction method for the on-line fluorimetric detection of natural vitamin K homologs was developed. In addition to its high sensitivity, the method features an interesting selectivity for the determination of these compounds in a complex matrix such as human serum. The formation of different photolysis products as a function of reaction conditions, and the optimalisation of a segmented flow reaction system will be discussed. The results indicate that the method is 4 times more sensitive than UV detection, and at least as selective as fluorescence detection after post-column chemical reduction.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02261609
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