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  • 1
    Electronic Resource
    Electronic Resource
    An asexual fungus has the potential for sexual development (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 60-68 
    Details
    ISSN: 1617-4623
    Keywords: Idiomorph ; DNA binding ; Mating type ; Bipolaris sacchari ; Cochliobolus heterostrophus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The availability of cloned genes that control sexual reproduction (mating type genes) in higher fungi has allowed us to consider the causes of failure to mate in asexual fungi. We report here that the asexual fungusBipolaris sacchari has a homolog of theMAT-2 gene of its sexual ascomycete relativeCochliobolus heterostrophus. TheB. sacchari MAT-2 sequence is highly similar to that ofC. heterostrophus MAT-2 and, in fact, functions in transgenicC. heterostrophus. Thus, the asexual nature ofB. sacchari is not due to absence or mutation ofMAT. When either of theC. heterostrophus MAT genes was transformed intoB. sacchari, the recipient could neither self nor cross with otherB. sacchari strains, in contrast to transgenicC. heterostrophus strains which can do both. Persistent asexuality ofB. sacchari, in spite of the presence of complementary functionalMAT genes, suggests that this fungus lacks genes other thanMAT which are essential for mating. Notably, the transgenicB. sacchari strains were sometimes able to initiate, but not complete, sexual development in interspecific pairings withC. heterostrophus. Transcript analysis showed that theB. sacchari MAT-2 gene is expressed in transgenicC. heterostrophus and that theC. heterostrophus MAT genes are expressed in transgenicB. sacchari. No transcript of the nativeB. sacchari MAT-2 gene was detected under any growth condition tested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02174345
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  • 2
    Electronic Resource
    Electronic Resource
    An asexual fungus has the potential for sexual development (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 60-68 
    Details
    ISSN: 1617-4623
    Keywords: Key words Idiomorph ; DNA binding ; Mating type ; Bipolaris sacchari ; Cochliobolus heterostrophus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The availability of cloned genes that control sexual reproducti on (mating type genes) in higher fungi has allowed us to consider the causes of failure to mate in asexual fungi. We report here that the asexual fungus Bipolaris sacchari has a homolog of the MAT-2 gene of its sexual ascomycete relative Cochliobolus heterostrophus. The B. sacchari MAT-2 sequence is highly similar to that of C. heterostrophus MAT-2 and, in fact, functions in transgenic C. heterostrophus. Thus, the asexual nature of B. sacchari is not due to absence or mutation of MAT. When either of the C. heterostrophus MAT genes was transformed into B. sacchari, the recipient could neither self nor cross with other B. sacchari strains, in contrast to transgenic C. heterostrophus strains which can do both. Persistent asexuality of B. sacchari, in spite of the presence of complementary functional MAT genes, suggests that this fungus lacks genes other than MAT which are essential for mating. Notably, the transgenic B. sacchari strains were sometimes able to initiate, but not complete, sexual development in interspecific pairings with C. heterostrophus. Transcript analysis showed that the B. sacchari MAT-2 gene is expressed in transgenic C. heterostrophus and that the C. heterostrophus MAT genes are expressed in transgenic B. sacchari. No transcript of the native B. sacchari MAT-2 gene was detected under any growth condition tested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02174345
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  • 3
    Electronic Resource
    Electronic Resource
    Many-electron-wavepackets method (1996)
    New York, NY : Wiley-Blackwell
    International Journal of Quantum Chemistry 60 (1996), S. 1291-1301 
    Details
    ISSN: 0020-7608
    Keywords: Computational Chemistry and Molecular Modeling ; Atomic, Molecular and Optical Physics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: We present a computational scheme to study the dynamics of many-electrons in molecular systems by wavepackets method. Several approaches to calculation of nonlinear optical properties for molecules under time-independent or time-dependent external electric fields are presented. Some simple examples of one-dimensional two- or three-electron systems are demonstrated concretely. Implications of these results are discussed in relation to the validity of the many electron wavepackets (MEWP) method. © 1996 John Wiley & Sons, Inc.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Instability in chemical bonds. II. Theoretical studies of exchange-coupled open-shell systems (1993)
    New York, NY : Wiley-Blackwell
    International Journal of Quantum Chemistry 48 (1993), S. 501-515 
    Details
    ISSN: 0020-7608
    Keywords: Computational Chemistry and Molecular Modeling ; Atomic, Molecular and Optical Physics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The electronic and geometrical structures of exchange-coupled open-shell systems such as bis(methylene) methane and the chromium-methylene cation were investigated by the molecular orbital and its extended methods: RHF, UHF, UNO-CAS, RMP, UMP, PUMP, APUMP, UCCSD(T), UNO-CI and QCISD(T). The fully optimized geometries of these species by the energy gradients of UMPn solutions are consistent with the experimental values. The total energies of the lowest and highest spin states were calculated in order to elucidate the effective exchange integrals in the Heisenberg model for methylene and bis(methylene) methane. The spin projection is necessary for the UHF-based wavefunctions in the lowest spin state of the species. The binding energies for the chromium cation-methylene double bond were calculated by both spin-unprojected and -projected methods. It was found that the binding energy between Cr+ and 3CH2 by APUHF QCISD(T) is in accord with the experimental value. © 1993 John Wiley & Sons, Inc.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/qua.560480848
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  • 5
    Electronic Resource
    Electronic Resource
    Nonadiabatic treatment of molecular systems by the wavepackets method (1996)
    New York, NY : Wiley-Blackwell
    International Journal of Quantum Chemistry 60 (1996), S. 1261-1270 
    Details
    ISSN: 0020-7608
    Keywords: Computational Chemistry and Molecular Modeling ; Atomic, Molecular and Optical Physics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: We have already developed the many-electron wavepackets (MEWP) method in order to study the dynamics and electronic structure of molecular systems. We extended the MEWP method to study the nonadiabatic effects and formulated a nonadiabatic molecular theory, where both electron and nucleus are treated equivalently. Then we applied our method to the isotope series of hydrogen molecule i.e., H2, HD, and D2, and calculated the total energy and the average distance between nucleus-nucleus, electron-electron, and nucleus-electron in order to analyze numerically the nonadiabatic effect in the molecule. Finally we calculated the real-time evolution of the polarization by means of Chebyshev scheme; and by Fourier transforming this, we found out the excitation spectrum of the system, which corresponds to the electronic excitation and the nuclear vibrational frequency. © 1996 John Wiley & Sons, Inc.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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