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  • Astrophysics  (1)
  • Bowman-Birk inhibitor  (1)
  • Dialysis  (1)
  • Ovariectomy  (1)
  • 1
    ISSN: 1573-4943
    Keywords: Bowman-Birk inhibitor ; microcalorimetry ; enthalpy ; fluorescence polarization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The binding of α-chymotrypsin to black-eyed pea trypsin/chymotrypsin inhibitor (BTCI) has been studied using the inhibitory activity against the enzyme and the formation of the complex enzyme/inhibitor followed by measurements of fluorescence polarization. Apparent equilibrium constants were estimated for several temperatures and the values obtained range from 0.32 × 107 to 1.36 × 107 M−1. The following values were found from van't Hoff plots: Δ H vh ° = 10.8 kcal mol-1 (from inhibitory assays) and 11.1 kcal mol−1 (from fluorescence polarization); ΔS° = 67.9 and = 67.8 kcal K−1 mol−1, respectively. Calorimetric binding enthalpy was determined (corrected for the ionization heat of the buffer) and the resulting value was ΔH cal ° = 4.9 kcal mol-1. These results indicate that the binding of chymotrypsin to BTCI is an entropically driven process.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 238 (1984), S. 559-564 
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Macrophages ; Ovariectomy ; Rat ; Estradiol administration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Macrophages of the adrenal cortex were studied in normal male and female, ovariectomized and estradiol-injected rats. In normal male rats few macrophages with numerous granules were observed in the zona fasciculatazona reticularis border, and in the zona reticularis. Granules, identified as lysosomes, were limited by a single membrane with a heterogeneous matrix; they exhibited acid phosphatase- and aminotriazole-resistant peroxidatic activities. A larger number of macrophages had identical distributions in normal female rats. In ovariectomized and estradiol-injected rats the number and distribution of adrenal macrophages were similar to those in normal females; however, in spayed animals the number of these cells in the zona reticularis was higher than in the other experimental groups. Lysosomes in macrophages of treated animals were more numerous and their contents more complex than in normal male animals. These results indicate that the adrenal macrophage system is stimulated in experimental conditions involving high levels of circulating estrogens.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Medical & biological engineering & computing 18 (1980), S. 765-771 
    ISSN: 1741-0444
    Keywords: Dialysis ; Dialyser reuse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Because of the high cost of dialysis, many centres have developed methods of cleaning diaylser cartridges so that they may be reused. This paper describes a machine which cleans dialyser cartridges automatically. The main difference between this machine and others reported in the literature is its ability to test automatically for leaks or obstruction and sound an alarm if the dialyser is unsuitable for reuse after the washing process. The development of these tests is decribed in detail. The incorporation of these tests into the alarm system of the machine will make the reuse of dialysers simpler, safer and more consistent.
    Type of Medium: Electronic Resource
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  • 4
    Publication Date: 2019-07-13
    Description: A novel laboratory experimental design is described that will investigate the processing of dust grains in astrophysical shocks. Dust is a ubiquitous ingredient in the interstellar medium (ISM) of galaxies; however, its evolutionary cycle is still poorly understood. Especially shrouded in mystery is the efficiency of grain destruction by astrophysical shocks generated by expanding supernova remnants. While the evolution of these remnants is fairly well understood, the grain destruction efficiency in these shocks is largely unknown. The experiments described herein will fill this knowledge gap by studying the dust destruction efficiencies for shock velocities in the range of approximately 10-30 kilometers per second (microns per nanosecond), at which most of the grain destruction and processing in the ISM takes place. The experiments focus on the study of grain-grain collisions by accelerating small (1 millimeter) dust particles into a large (approximately 5-10 millimeter diameter) population; this simulates the astrophysical system well in that the more numerous, small grains impact and collide with the large population. Facilities that combine the versatility of high-power optical lasers with the diagnostic capabilities of X-ray free-electron lasers, e.g., the Matter in Extreme Conditions instrument at the SLAC (originally named Stanford Linear Accelerator Center) National Accelerator Laboratory, provide an ideal laboratory environment to create and diagnose dust destruction by astrophysically relevant shocks at the micron scale.
    Keywords: Astrophysics
    Type: GSFC-E-DAA-TN67044 , High Power Laser Science and Engineering (ISSN 2095-4719) (e-ISSN 2052-3289); 6; e39
    Format: application/pdf
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