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  • 1
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 18 (1991), S. 189-203 
    ISSN: 0886-1544
    Keywords: microtubules ; isotubulins ; actin ; brine shrimp ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: In many differentiated cells, posttranslationally modified tubulins exhibit restricted subcellular distribution, leading to the proposal that they are required for the production and maintenance of polarity. To study this possibility, we used immunological approaches to examine tubulin isoforms in developing Artemia larvae and to determine their location in several types of cells within the organism. The amount of tubulin in relation to total protein remained relatively constant during early larval development while detyrosinated tubulin increased, an event correlated with the differentiation of larval gut muscle cells. Except for epidermal cells of the developing thorax, each type of cell within the Artemia larvae exhibited characteristic staining patterns which were very similar for each antitubulin antibody. Within epidermal cells, microtubules containing acetylated tubulin appeared patchy or punctate in their distribution, an image not seen with the other antibodies. In most polarized cells, staining for tubulin and actin colocalized in discrete areas, demonstrating enrichment of both proteins within the same cellular compartment and suggesting functional interactions. Mitotic figures were stained with qualitatively equal intensity by all of the antitubulin antibodies, but asters were not observed. Midbodies were intensely stained with phalloidin as well as the antibodies to tubulin. It was clear that microtubules exhibited a preferential localization in cells of Artemia but in no case was a tubulin isoform found exclusively in one area of a cell. The results support the contention that microtubules influence the organization of polarized cell structure and function but they do not permit the conclusion that this capability is dependent on the localization of posttranslationally modified tubulins to restricted subcellular positions.
    Additional Material: 8 Ill.
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 26 (1993), S. 77-87 
    ISSN: 0886-1544
    Keywords: cell surface iodination ; intermediate filaments ; cell surface keratins ; keratins ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Keratins are a subgroup of cytoskeletal intermediate filament proteins found in most epithelial cells. Some reports have suggested that keratins may be found on the cell surface as well as their well-accepted cytoskeletal location. A major part of the evidence in the interpretation of cell surface expression of keratins is cell surface radioiodination. Here we show that lactoperoxidase-catalyzed iodination of colonic and breast tissue culture cells results in radiolabeling of the keratins when cells are manipulated. No labeling of keratins is detected when cells are labeled directly on the tissue culture dish. A similar result was obtained when intact cells were biotinylated using water-soluble sulfo-NHS-biotin. Partitioning of the keratins to a soluble and an insoluble pool after “cell surface” 125I-labeling showed that both pools became iodinated. Indirect immunofluorescence showed that binding of a panel of anti-keratin antibodies to intact epithelial cells occurs only on the cells that are more adherent, which are the cells that require longer manipulation to remove from the tissue culture dish. Taken together, our results indicate that the reported expression of cell surface keratins in some cells likely reflects intracellular keratins. In addition, the method of epithelial cell handling can dramatically alter the leakiness of cell surface iodination techniques. © 1993 Wiley-Liss, Inc.
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  • 3
    Electronic Resource
    Electronic Resource
    Philadelphia : Wiley-Blackwell
    Journal of Cellular and Comparative Physiology 39 (1952), S. 261-268 
    ISSN: 0095-9898
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Additional Material: 3 Tab.
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  • 4
    ISSN: 0749-503X
    Keywords: Saccharomyces cerevisiae ; yeast ; gene disruption ; S288C ; bacteria-yeast shuttle vectors ; auxotrophic markers ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: A set of yeast strains based on Saccharomyces cerevisiae S288C in which commonly used selectable marker genes are deleted by design based on the yeast genome sequence has been constructed and analysed. These strains minimize or eliminate the homology to the corresponding marker genes in commonly used vectors without significantly affecting adjacent gene expression. Because the homology between commonly used auxotrophic marker gene segments and genomic sequences has been largely or completely abolished, these strains will also reduce plasmid integration events which can interfere with a wide variety of molecular genetic applications. We also report the construction of new members of the pRS400 series of vectors, containing the kanMX, ADE2 and MET15 genes. © 1998 John Wiley & Sons, Ltd.
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  • 5
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    In:  http://aquaticcommons.org/id/eprint/15181 | 403 | 2014-05-29 07:51:01 | 15181 | United States National Marine Fisheries Service
    Publication Date: 2021-07-04
    Keywords: Fisheries ; Management
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 923-932
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  • 6
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    California Department of Fish and Game Marine Resources Division | Monterey, CA
    In:  http://aquaticcommons.org/id/eprint/109 | 8 | 2011-09-29 22:34:32 | 109 | California Department of Fish and Game
    Publication Date: 2021-06-26
    Description: In 1992 fishery technicians sampled 230 commercial passenger fishing vessel (CPFV) trips targeting rockfish and lingcod from the port areas of Fort Bragg, Bodega Bay, San Francisco, Monterey, and Morro Bay. The skippers of 44 vessels, and 2,190 anglers, cooperated in the study. Species composition by port area and month, catch-per-unit-effort, mean length, and length frequency of lingcod and the 18 most frequently observed rockfish species are presented, as well as fishing effort relative to time, depth, and distance from port. Total catch estimates based on unadjusted and adjusted logbook records are summarized.Average catch of kept fish per angler day was 12.6 and average catch of kept fish per angler hour was 4.0. A continuing trend of an increasing frequency of trips to deep (〉 40 fm) locations was observed in the Bodega Bay, San Francisco, and Monterey areas. Bodega Bay and San Francisco showed the highest frequency of trips to distant locations.Sixty species comprised of 29,731 fish were observed caught during the study. Rockfish comprised 93.5% by number of the total observed catch. The five most frequently observedspecies were blue, yellowtail, widow and rosy rockfishes, and bocaccio, with lingcod ranking eighth.CPFV angler success, as determined by catch per angler hour, generally increased in all ports in 1992 compared to previous 1988-91 data (Reilly et al. 1993). However, port-specific areas of major concern were identified for chilipepper, lingcod, and black rockfish, and to alesser extent brown, canary, vermilion, yelloweye, widow and greenspotted rockfishes. These areas of concern included steadily declining catch rate, steadily declining mean length, and/or a high percentage of sexually immature fish in the sampled catch.Recent sampling of the commercial hook-and-line fishery in northern and central California indicates that most rockfishes taken by CPFV anglers are also harvested commercially. (105pp.)
    Keywords: Management ; Fisheries ; rockfish ; lingcod ; sport fishing ; California
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
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  • 7
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    California Department of Fish and Game Marine Region | Monterey, CA
    In:  http://aquaticcommons.org/id/eprint/107 | 8 | 2011-09-29 22:34:23 | 107 | California Department of Fish and Game
    Publication Date: 2021-06-26
    Description: The Central California Marine Sport Fish Project has been collecting angler catch data on board Commercial Passenger Fishing Vessels (CPFVs) fishing for rockfish or lingcod since 1987. The program depends on the voluntary cooperation of CPFV owners and operators. This fifth report in a series presents data collected in 1995, refers to historical data from 1987 to 1994, and documentstrends in species composition, angler effort, catch per unit effort (CPUE), and, for selected species, mean length, and length frequency.Angler catches on board central and northern California CPFVs were sampled from 12 ports, ranging from Fort Bragg in the north to Port San Luis (Avila Beach) in the south. Technicians observed a total of 1829 anglers fishing on 218 CPFV trips. These observed anglers caught 26,197fish of which samplers determined 22,888 were kept. Over 62% of these fish were caught at Monterey or Morro Bay area ports. Only 18 of 55 species comprised at least one percent of the catch. The top ten species in order of abundance were yellowtail, blue, olive, and rosy rockfishes, lingcod, and canary, widow, gopher, starry, and vermilion rockfishes. Blue and yellowtail rockfishes together comprised approximately 47% of the observed catch. Overall, rockfishes represented 35 species or 64% of the 55 identified species. By number, rockfishes comprised 91.9 % of the observed catch.All CPUE and length data collected since 1987 were partitioned into six location groups for each port area, based on a combination of location, bottom depth, and distance from the nearest port. This allowed examination of indicator trends without potential biases due to non-random trip selection or ontogenetic changes in depth distribution for certain rockfishes. Results indicate thatthe two primary species in the northern and central California CPFV fishery, blue and yellowtailrockfishes (accounting for 47% of all observed fish in 1995), are in reasonably good condition with no steady declines in either average catch per angler hour or mean length during the last 8 years. The primary species of concern are mainly shallow-water species impacted by a recently expanded commercial hook-and-line fishery or deep-water species (chilipepper and bocaccio) which are fished intensively by the commercial industry and have experienced recent statewide stock declines.Estimated total rockfish catch, adjusted by logbook compliance rates and on board sampling data, has declined significantly in all port areas since 1992. This is largely attributable to increased recreational fishing effort for salmon as well as coast-wide stock declines in several important commercially fished species. (112pp.)
    Keywords: Management ; Fisheries ; rockfish ; lingcod ; sport fishing ; California
    Repository Name: AquaDocs
    Type: monograph
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  • 8
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    California Department of Fish and Game Marine Resources Division | Monterey, CA
    In:  http://aquaticcommons.org/id/eprint/389 | 8 | 2011-09-29 22:07:57 | 389 | California Department of Fish and Game
    Publication Date: 2021-06-29
    Description: The Central California Marine Sport Fish Project has been collecting angler catch data on board Commercial Passenger Fishing Vessels (CPFVs) fishing for rockfish or lingcod since 1987. The program depends on the voluntary cooperation of CPFV owners and operators. This fourth report in a series presents data collected in 1994, refers to historical data from 1987 to 1993, and documents trends in species composition, angler effort, catch per unit effort (CPUE), and, for selected species, mean length and length frequency.Angler catches on board central and northern California CPFVs were sampled from 14 ports, ranging from Crescent City in the north to Port San Luis (Avila Beach) in the south. Technicians observed a total of 2053 anglers fishing on 237CPFV trips. These observed anglers caught 24,731 fish of which samplers determined 22,615 were kept. Over 63% of these fish were caught at Monterey or Morro Bay area ports.Only 18 of the 53 species each comprised at least one percent of the catch. The top ten species in order of abundance were blue, yellowtail, rosy, chilipepper, canary, and black rockfishes, lingcod, and widow, starry and greenspotted rockfishes. Blue and yellowtail rockfishestogether comprised approximately 44% of the observed catch. Overall, rockfishes represented 32 species or 60% of the 53 identified species.In general, 1994 data showed continued evidence of sustainability of the CPFV rockfish fishery with a few exceptions for some species in each port area sampled. We identified areas of concern for three nearshore species (black, blue, and brown rockfishes), three offshore species(bocaccio, chilipepper, and widow rockfish), and two wide-depth range species (canary and yelloweye rockfishes). Declining trends in CPAH in at least one port area were identified for each of these species except black and bluerockfishes, some of which may be related to natural variability in year class strength or stock movement. Mean length decreased in at least one port area for black, blue, brown, and canary rockfishes and chilipepper, although for black rockfish and chilipepper this was attributed toincreased recruitment of juveniles.Positive trends identified included increased CPAH for black rockfish in the San Francisco and Monterey areas, for canary rockfish in the San Francisco area, and for brown rockfish in the Morro Bay area.Total northern and central California CPFV catch estimates, based on logbook data and adjusted by sampling data and compliance rates, indicated that port area-specific decreases have occurred since 1988 for yellowtail rockfish and lingcod (San Francisco), chilipepper (Monterey),and blue rockfish (Monterey and Morro Bay).The competing influences of the nearshore commercial hook-and-line fishery were apparent when comparing relative species composition to that of the CPFV fishery. Declines in CPAH in the CPFV fishery for some shallow water species, such as brown rockfish in the Bodega Bay area, may be related to increased commercial fishing effort. (98pp.)
    Keywords: Management ; Fisheries ; Biology ; Lingcod ; Ophiodon elongatus ; blue rockfish ; Sebastes mystinus ; Yellowtail Rockfish ; Sebastes flavidus ; rosy rockfish ; Sebastes rosaceus ; chilipepper rockfish ; Sebastes goodei ; canary rockfish ; Sebastes pinniger ; Black rockfish ; Sebastes melanops ; widow rockfish ; Sebastes entomelas ; Starry rockfish ; Sebastes constellatus ; Greenspotted rockfish ; Sebastes chlorostictus ; sport fishing
    Repository Name: AquaDocs
    Type: monograph
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  • 9
    Publication Date: 2019-07-13
    Description: We present Spitzer 3.6 and 4.5 micrometer photometry and positions for a sample of 1510 brown dwarf candidates identified by the Wide-field Infrared Survey Explorer (WISE) all-sky survey. Of these, 166 have been spectroscopically classified as objects with spectral types M(1), L(7), T(146), and Y(12). Sixteen other objects are non-(sub)stellar in nature. The remainder are most likely distant L and T dwarfs lacking spectroscopic verification, other Y dwarf candidates still awaiting follow-up, and assorted other objects whose Spitzer photometry reveals them to be background sources. We present a catalog of Spitzer photometry for all astrophysical sources identified in these fields and use this catalog to identify seven fainter (4.5 m to approximately 17.0 mag) brown dwarf candidates, which are possibly wide-field companions to the original WISE sources. To test this hypothesis, we use a sample of 919 Spitzer observations around WISE-selected high-redshift hyper-luminous infrared galaxy candidates. For this control sample, we find another six brown dwarf candidates, suggesting that the seven companion candidates are not physically associated. In fact, only one of these seven Spitzer brown dwarf candidates has a photometric distance estimate consistent with being a companion to the WISE brown dwarf candidate. Other than this, there is no evidence for any widely separated (greater than 20 AU) ultra-cool binaries. As an adjunct to this paper, we make available a source catalog of 7.33 x 10(exp 5) objects detected in all of these Spitzer follow-up fields for use by the astronomical community. The complete catalog includes the Spitzer 3.6 and 4.5 m photometry, along with positionally matched B and R photometry from USNO-B; J, H, and Ks photometry from Two Micron All-Sky Survey; and W1, W2, W3, and W4 photometry from the WISE all-sky catalog.
    Keywords: Astronomy
    Type: GSFC-E-DAA-TN6577 , The Astronomical Journal; 155; 5; 148
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  • 10
    Publication Date: 2019-07-13
    Description: We have used the Caltech Submillimeter Observatory (CSO) to follow-up a sample of Wide-field Infrared Survey Explorer (WISE) selected, hyperluminous galaxies, the so-called W1W2-dropout galaxies. This is a rare (approx.1000 all-sky) population of galaxies at high redshift (peaks at z = 2-3), which are faint or undetected by WISE at 3.4 and 4.6 microns, yet are clearly detected at 12 and 22 microns. The optical spectra of most of these galaxies show significant active galactic nucleus activity. We observed 14 high-redshift (z 〉 1.7) W1W2-dropout galaxies with SHARC-II at 350-850 microns, with nine detections, and observed 18 with Bolocam at 1.1 mm, with five detections. Warm Spitzer follow-up of 25 targets at 3.6 and 4.5 microns, as well as optical spectra of 12 targets, are also presented in the paper. Combining WISE data with observations from warm Spitzer and CSO, we constructed their mid-IR to millimeter spectral energy distributions (SEDs). These SEDs have a consistent shape, showing significantly higher mid-IR to submillimeter ratios than other galaxy templates, suggesting a hotter dust temperature.We estimate their dust temperatures to be 60 C120 K using a single-temperature model. Their infrared luminosities are well over 10(exp 13) Stellar Luminosity. These SEDs are not well fitted with existing galaxy templates, suggesting they are a new population with very high luminosity and hot dust. They are likely among the most luminous galaxies in the universe.We argue that they are extreme cases of luminous, hot dust-obscured galaxies (DOGs), possibly representing a short evolutionary phase during galaxy merging and evolution. A better understanding of their long-wavelength properties needs ALMA as well as Herschel data.
    Keywords: Astronomy
    Type: GSFC-E-DAA-TN-6869 , The Astrophysical Journal; 756; 1; 96
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