Publication Date:
2021-07-12
Description:
In this study the genetic diversity of wild Caspian trout (Salmo trutta caspius) in the Sardabroud and Astara Rivers was evaluated using D-Loop region sequencing. For this purpose, 35 specimens of adult Caspian brown trout were collected from these rivers in the Mazandarn and Gilan Provinces in fall and winter 2011. Approximately 3-5 g of soft and fresh fin tissue was isolated and fixed in ethanol 96% and then transferred to the Caspian Sea Ecology Research Center Genetics Laboratory in Sari, Iran. Genomic DNA from the samples was extracted using Ammonium Acetate Method. The quality and quantity of the extracted DNA were assessed by spectrophotometer and agarose gel (1%) electrophoresis. Polymerase Chain Reaction (PCR) was performed on the target DNA using a primers sequence D-Loop region of mtDNA molecule. Then the product was purified and DNA sequencing was carried out using chain termination method. The D-Loop region of Caspian trout contained 654 bp. Data were analyzed using Bio-Edit, DnaSP, Arlequin and Mega software. 20 and 15 haplotypes was observed in Sardabrud and Astara River. The DNA sequence of one of them was recorded in Gene Bank with numbers KC991027 and KF015727. 223 and 240 polymorphic loci were detected in Sardabrud and Astara River that all of them were out of Hardy-Weinberg equilibrium (p〈0.05). Average nucleotide and haplotype diversity were 0.127±0.067, 1.000±0.005 in Sardabrud River and 0.118±0.063 and 1.000±0.005 in Astara River.
Keywords:
Biology
;
Fisheries
;
Genetic diversity
;
Salmo trutta caspius
;
Sardabrud
;
Astara
;
Sequencing
;
Sardabrud River
;
Astara River
;
Iran
Repository Name:
AquaDocs
Type:
article
,
TRUE
Format:
application/pdf
Format:
application/pdf
Format:
352-365
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