ISSN:
1573-5028
Keywords:
Arthrobacter globiformis
;
choline oxidase
;
codA gene
;
glycinebetaine
;
salt tolerance
;
Synechococcus
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Choline oxidase, isolated from the soil bacterium Arthrobacter globiformis, converts choline to glycinebetaine (N-trimethylglycine) without a requirement for any cofactors. The gene for this enzyme, designated codA, was cloned and introduced into the cyanobacterium Synechococcus sp. PCC 7942. The codA gene was experssed under the control of a strong constitutive promoter, and the transformed cells accumulated glycinebetaine at intracellular levels of 60–80 mM. Consequently the cells acquired tolerance to salt stress, as evaluated in terms of growth, accumulation of chlorophyll and photosynthetic activity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00014964
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