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  • 1
    ISSN: 1432-203X
    Keywords: Key wordsM. truncatula ; M. falcata ; MsEnod12A ; MsEnod12B ; Srglb3
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We describe a simple and efficient protocol for regeneration-transformation of two diploid Medicago lines: the annual M. truncatula R108-1(c3) and the perennial M. sativa ssp. falcata (L.) Arcangeli PI.564263 selected previously as highly embryogenic genotypes. Here, embryo regeneration of R108-1 to complete plants was further improved by three successive in vitro regeneration cycles resulting in the line R108-1(c3). Agrobacterium tumefaciens-mediated transformation of leaf explants was carried out with promoter-gus constructs of two early nodulins (MsEnod12A and MsEnod12B) and one late nodulin (Srglb3). The transgenic plants thus produced on all explants within 3–4 months remained diploid and were fertile. This protocol appears to be the most efficient and fastest reported so far for leguminous plants.
    Type of Medium: Electronic Resource
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  • 2
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    In:  Blumea: Biodiversity, Evolution and Biogeography of Plants vol. 68, pp. 39-48
    Publication Date: 2024-03-19
    Description: Two species of the Araceae family, Typhonium praelongum (having tuber of blunt cone shape, very long \n(about 30 cm) sterile appendix of the spadix, horizontal yellow-orange staminodes with tops turning upwards and \na glossy entirely papillate upper surface of the simple leaf blade) and Amorphophallus opalinus (having narrow \nrhizome-like tuber with enlarged basal part, staminodes at the base of male zone of the spadix, narrow spathe, \nlarge, broadly-conical with rounded apex, opalescent stigmas and white, one-seeded berries), are described as \nspecies new to science from South Vietnam. Detailed morphological descriptions, colour plates of the species and \ntheir habitats, phenology, distribution map, provisional conservation status, taxonomic comments and overview of \nthe genera Typhonium and Amorphophallus in the Flora of Vietnam are provided.
    Keywords: Amorphophallus ; Araceae ; Areae ; Binh Thuan Province ; Thomsonieae ; Typhonium ; Vietnam
    Repository Name: National Museum of Natural History, Netherlands
    Type: info:eu-repo/semantics/article
    Format: application/pdf
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