Publication Date:
2021-07-16
Description:
The study was done in Nutrition and Live Food Station was located in Bandar Anzali Ghaziyan. Juveniles weighted average 0.5, 1, 2.5, 5, 10, 15 and 20 g were randomly selected in three water conditions with a salinity of 11 ppt (Caspian sea water), water 7 ppt and freshwater (with three replicates per group) were included. At intervals of 0, 3, 6, 12, 24, 72, 168, 240 hours, blood samples were heparinized capillary tubes by caudal juveniles and ion concentrations Mg, Ca,Cl using the spectrophotometer and sodium and potassium ions with Flaym photometry (flame photometric), the osmotic pressure of blood plasma by osmometer and cortisol levels were measured by RIA method. To study the microstructure of gill and kidney tissue for each treatment , tissue samples by classical histological methods and stained with hematoxylin - eosin slides were prepared. The frequency and location of the enzyme Na^+, K^+ - ATPase and chloride cells with immunohistochemical localization was performed. Studies micrometric gill chloride cells and renal glomerular networks by software Image tool (version 2.0) was performed. Measurement of enzyme Na^+, K^+-ATPase, by Zaugg (1982) method was conducted. Data analyzed by one-way ANOVA (Oneway ANOVA) with Tukey's test was performed. Overall, the results of measuring ions and osmotic pressure on the tenth day of treatment, the osmotic potential juveniles 2.5, 5, 10, 20 gr in Caspian sea water and all groups except the 0.5 in water of 7 ppt confirmed. But in case of unfavorable conditions for the release in estuaries river and river, fish with weight 1 to 3 release directly to beach (where the salinity is 7 grams per liter) and fishes with weight from 10 to 20 gr to sea. Although suitable river conditions necessary condition for release of juveniles in riverine areas to adaptation juveniles occur gradually.
Keywords:
Aquaculture
;
Iran
;
Caspian Sea
;
Rutilus frisii kutum
;
Osmolarity
;
Suitable size
;
Release
Repository Name:
AquaDocs
Type:
monograph
Format:
application/pdf
Format:
application/pdf
Format:
54
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