ISSN:
1573-675X
Keywords:
Apoptosis
;
β-IMAGER
;
dengue virus
;
DNA fragmentation
;
radioimager μ-IMAGER
;
rabie virus.
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract DNA fragmentation is a key feature of the degradation phase of apoptosis. In this work we have developed an assay, based on radioimager (β-IMAGER and μ-IMAGER) quantification of radioactive nick end labelling (RANEL), which is quantitative, rapid and sensitive to study in vitro and in vivo induced apoptosis. To establish the technique, in vitro apoptosis of T cell lines was induced by stimulation of the Fas receptor; cells were labelled using TdT-mediated [α-33P] dCTP nick end labelling, after which then radioactivity was quantified using a β-IMAGER. We have also shown that the RANEL method can be applied to the quantification and visualisation, by μ-IMAGER analysis, of liver tissue sections from mouse Fas-induced fulminant hepatitis or from Dengue-1 virus infected individuals. Finally, this system has also been used to detect apoptosis induced by rabies virus in Jurkat T cells. These data have established a large field of application for the RANEL assay.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1009658522328
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