Publication Date:
1993-04-23
Description:
The CD45 protein is a transmembrane tyrosine phosphatase that is required for normal T cell receptor (TCR)-mediated signaling. A chimeric complementary DNA encoding the intracellular enzymatically active portion of murine CD45 preceded by a short amino-terminal sequence from p60c-src was transfected into CD45- T cells. Expression of this chimeric protein corrected most of the TCR signaling abnormalities observed in the absence of CD45, including TCR-mediated enhancement of tyrosine kinase activity and Ca2+ flux. Thus, the enzymatically active intracellular portion of CD45 is sufficient to allow TCR transmembrane signaling.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Volarevic, S -- Niklinska, B B -- Burns, C M -- June, C H -- Weissman, A M -- Ashwell, J D -- New York, N.Y. -- Science. 1993 Apr 23;260(5107):541-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Laboratory of Immune Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8475386" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Antigens, CD3/immunology
;
Antigens, CD45/genetics/*metabolism
;
Base Sequence
;
Calcium/metabolism
;
Cell Membrane/metabolism
;
Membrane Proteins/metabolism
;
Mice
;
Molecular Sequence Data
;
Phosphorylation
;
Protein-Tyrosine Kinases/metabolism
;
Receptors, Antigen, T-Cell/*metabolism
;
Recombinant Fusion Proteins/metabolism
;
*Signal Transduction
;
Tumor Cells, Cultured
;
Tyrosine/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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