Publication Date:
2007-10-06
Description:
Mounting a protective immune response is critically dependent on the orchestrated movement of cells within lymphoid organs. We report here the visualization, using major histocompatability complex class I tetramers, of the CD8-positive (CD8) T cell response in the spleens of mice to Listeria monocytogenes infection. A multistage pathway was revealed that included initial activation at the borders of the B and T cell zones followed by cluster formation with antigenpresenting cells leading to CD8 T cell exit to the red pulp via bridging channels. Strikingly, many memory CD8 T cells localized to the B cell zones and, when challenged, underwent rapid migration to the T cell zones where proliferation occurred, followed by egress via bridging channels in parallel with the primary response. Thus, the ability to track endogenous immune responses has uncovered both distinct and overlapping mechanisms and anatomical locations driving primary and secondary immune responses.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2846662/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2846662/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Khanna, Kamal M -- McNamara, Jeffery T -- Lefrancois, Leo -- AI41576/AI/NIAID NIH HHS/ -- AI56172/AI/NIAID NIH HHS/ -- DRG-1886-05/PHS HHS/ -- P01 AI056172/AI/NIAID NIH HHS/ -- P01 AI056172-05/AI/NIAID NIH HHS/ -- R01 AI041576/AI/NIAID NIH HHS/ -- R01 AI041576-06/AI/NIAID NIH HHS/ -- New York, N.Y. -- Science. 2007 Oct 5;318(5847):116-20.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Immunology, University of Connecticut, Farmington, CT 06030, U.S.A.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17916739" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Antigen-Presenting Cells/immunology
;
Antigens, CD/analysis
;
Antigens, CD8/analysis
;
B-Lymphocytes/immunology
;
CD8-Positive T-Lymphocytes/cytology/*immunology/physiology
;
Cell Movement
;
Dendritic Cells/immunology/physiology
;
Fluorescent Dyes
;
Histocompatibility Antigens Class I
;
*Immunologic Memory
;
Kinetics
;
Listeria monocytogenes/*immunology
;
Listeriosis/*immunology
;
Lymphocyte Activation
;
Mice
;
Mice, Inbred C57BL
;
Microscopy, Confocal
;
Receptors, Antigen, T-Cell/analysis
;
Spleen/cytology/*immunology
;
Staining and Labeling
;
T-Lymphocyte Subsets/cytology/immunology/physiology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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