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  • doubled haploid  (2)
  • Anther culture  (1)
  • Blackroot rot  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Effects of the Nicotiana debneyi black root rot resistance factor on agronomic and chemical traits in burley tobacco (1981)
    Springer
    Theoretical and applied genetics 60 (1981), S. 365-368 
    Details
    ISSN: 1432-2242
    Keywords: Disease resistance ; Isogenic lines ; Blackroot rot ; Thielaviopsis basicola ; Nicotiana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Lines isogenic or near isogenic for traits other than resistance to black root rot from Nicotiana debneyi were developed in eight cultivar backgrounds of burley tobacco (N. tabacum L.). For each cultivar background, a resistant and susceptible selection from the seventh backcross generation plus the recurrent parental cultivar were evaluated for ten agronomic and chemical traits. Resistant selections were statistically different from the susceptible entries for days to flower, total nitrogen content, and total alkaloid content. Also, resistant selections were consistently lower in yield, but the differences were statistically nonsignificant. Resistant selections were also taller in three families and in two families the resistant selections had wider leaves. Linkage of genetic material from N. debneyi with the resistance factor was suggested as the possible reason for differences between resistant and susceptible selections.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00264332
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  • 2
    Electronic Resource
    Electronic Resource
    A comparison of burley tobacco doubled-haploid lines with their source inbred cultivars (1982)
    Springer
    Theoretical and applied genetics 62 (1982), S. 69-74 
    Details
    ISSN: 1432-2242
    Keywords: Nicotiana tabacum L. ; Anther culture ; Haploids ; Diallel ; Reciprocal cross
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A doubled-haploid line was randomly derived from each of seven burley tobacco, Nicotiana tabacum L., cultivars using anther culture and leaf-midvein chromosomal doubling. The doubled-haploid lines were compared to their source inbred cultivars in two experiments for several agronomic and chemical characters. A consistent relationship between anther-derived materials and reduced vigor was not observed in these doubled-haploid lines. Significant differences between the doubled-haploid mean and their source cultivar mean were observed only for days to flower and total alkaloids. The doubled-haploid means were not significantly different from their source cultivar means for yield, plant height, and leaf number. In total, 26 of the 35 individual comparisons between a doubled-haploid line and its source cultivar were nonsignificant. Reciprocal crosses between the doubled-haploid lines and their source inbred cultivars also demonstrated no significant differences. The diallel progeny of the seven doubled-haploid lines showed a similar genetic performance to that of diallel progeny from conventional materials in previous studies. The differences observed between the doubled-haploid lines and their source inbred cultivars could be explained by residual heterozygosity. The performances of the doubled-haploid progeny could also be attributed to the presence of residual heterozygosity in the original cultivars. As tested by reciprocal crosses, cytoplasmic effects were not significantly involved in the performance of the double-dhaploid lines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276286
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  • 3
    Electronic Resource
    Electronic Resource
    Vigor and variation expressed by anther-derived doubled haploids of burley tobacco (Nicotiana tabacum L.). I. Comparison of sexual and doubled-haploid populations (1986)
    Springer
    Euphytica 35 (1986), S. 33-40 
    Details
    ISSN: 1573-5060
    Keywords: Nicotiana tabacum ; burley tobacco ; anther culture ; doubled haploid ; colchicine ; androgenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The genetic consequences of anther culture and chromosome-doubling techniques on burley tobacco (Nicotiana tabacum L.) were examined in this study. Three diploid populations, obtained from a burley tobacco inbred by conventional and anther-culture techniques, were compared. The first population consisted of 50 conventionally-selfed lines; the second population was made up of 35 doubled-haploid lines obtained from individual haploid plants by in vitro techniques (IVDH); and the third population consisted of 20 doubled-haploid lines whose chromosome complements had been doubled with colchicine (CDH). Comparisons of doubled-haploid lines with sexually-derived lines revealed significant differences for yield, maturity, leaf length, and alkaloid content. Yield reductions in the doubled-haploid populations averaged 8.5%. Significant differences observed between the IVDH and CDH populations indicate that the reported deleterious effects of colchicine contributed to the vigor reduction of doubled haploids. The anther derived lines in this study exhibited greater variation than did the sexual materials. This variation could provide useful variation for a breeding program. Variation exhibited by the sexual progeny of the highly inbred line, ‘Kentucky 16’, suggests that the differences among anther-derived materials are at least partially due to natural phenomena.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00028538
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  • 4
    Electronic Resource
    Electronic Resource
    Vigor and variation expressed by anther-derived doubled haploids of burley tobacco (Nicotiana tabacum L.). II. Evaluation of first- and second-cycle doubled haploids (1986)
    Springer
    Euphytica 35 (1986), S. 41-48 
    Details
    ISSN: 1573-5060
    Keywords: Nicotiana tabacum ; burley tobacco ; anther culture ; doubled haploid ; evaluation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary First- and second-cycle doubled haploids of burley tobacco (Nicotiana tabacum L.) were examined to ascertain the significance of source genotype on the performance of anther-derived material and to determine the magnitude of changes from the haploid-production and chromosome-doubling stages. The anther-derived lines evaluated in this study did not exhibit siginficant yield reductions. However, significant interactions between the cycle comparison and the source genotypes indicated that source genotype can affect the degree of vigor reduction observed. Significant variation was also observed among the second-cycle doubled haploids examined in this study. Partitioning of this variation indicates that most of it is due to alteration present at the haploid-production step instead of the chromosome-doubling step. In general, the variation present in the second-cycle doubled haploids was distributed around the values observed for the source first-cycle doubled haploids. Even though unexpected variation is often observed in anther-derived materials, these results suggest that reduced vigor is not intrinsically associated with androgenetic lines of burley tobacco. The most plausible explanations for the variation and vigor losses in anther-derived lines propose that natural phenomena, given the opportunity for expression via anther culture, are responsible. Spontaneous variants present in the gametic pool of inbreds or first-cycle doubled haploids offer one possible explanation for the performance observed in anther-derived lines of tobacco.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00028539
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