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  • 1
    Electronic Resource
    Electronic Resource
    Ultrastructural observations of growth hormone (STH) cells of anterior pituitary glands of partially hepatectomized rats (1975)
    Springer
    Cell & tissue research 163 (1975), S. 343-351 
    Details
    ISSN: 1432-0878
    Keywords: Growth-hormone cell ; Anterior pituitary ; Hepatectomy, rat ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ultrastructural changes in pituitary growth-hormone cells were observed in partially hepatectomized rats. The hepatectomies were carried out during the afternoon after 3 p.m. The animals were sacrificed by decapitation at midnight at intervals of 32, 80, and 104 hours after the operation. The principal changes in the growth-hormone cells of anterior pituitary glands of partially hepatectomized rats were: (1) increased numbers of secretory granules in exocytosis, (2) increased numbers of microtubules, and (3) enlargement of endoplasmic reticulum and occurrence of dilated cisternae of the endoplasmic reticula. Many growth-hormone cells contained a reduced number of secretory granules. Exocytosis of growth-hormone granules was more frequently observed in animals sacrificed at 32 hours after the operation than in those killed at 80 or 104 hours after surgery. The above results in which appearance of numerous microtubules and active secretory granule extrusion in the growth-hormone cells were observed after hepatectomy indicate that ultrastructure of growth-hormone cells and growth hormone secretion were markedly stimulated by the operation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219468
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  • 2
    Electronic Resource
    Electronic Resource
    Unique features of secretory granules observed in the pituitary growth hormone-secreting (GH) cells of the musk shrew (Suncus murinus L.) (1988)
    Springer
    Cell & tissue research 251 (1988), S. 111-116 
    Details
    ISSN: 1432-0878
    Keywords: Secretory granules ; GH cells ; Anterior pituitary ; Musk shrew ; Suncus murinus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Unique rod-shaped secretory granules were observed among oval or spherical secretory granules in GH cells of the anterior pituitary gland of musk shrew using the protein A-gold procedure combined with electron microscopy. The rod-shaped and spherical secretory granules were both immunoreactive by the immuno-gold method using antiserum to sheep GH. The rod-shaped secretory granules, which seem to be formed directly from the Golgi vesicles, extend from several hundred to several thousand nm in length. They often touch each other and fuse. The spherical secretory granules are also unique in that they may also fuse with loss of dense contents to leave “empty” circular membrane profiles in the cytoplasm. Both the rodshaped and spherical secretory granules are secreted from the cell by exocytosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215454
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  • 3
    Electronic Resource
    Electronic Resource
    Effects of rat serum on gonadotrophin release in vitro (1983)
    Springer
    Cell & tissue research 229 (1983), S. 197-205 
    Details
    ISSN: 1432-0878
    Keywords: Anterior pituitary ; Gonadotrophs ; Gonadotrophin release ; Serum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Gonadotrophin release from a monolayer of cultured pituitary cells from the rat was increased by the addition of fresh rat serum or rat serum albumin to the culture medium. Similar effects were obtained when horse, chicken, lamb or porcine serum was used. Fetal bovine serum or bovine serum albumin showed a stimulatory effect on FSH and LH release but, unlike the case with rat serum, a dose-response release could not be demonstrated. In addition, fresh rat serum caused the accumulation of secretory granules in the gonadotrophs. The addition of LHRH to the culture medium also stimulated gonadotrophin release in a dose-response dependent manner. The dependence of the gonadotrophs on LHRH was markedly increased by the addition of 50% fresh rat serum to the culture medium. These results indicate that fresh rat serum contains some unknown factor(s) which 1) stimulates gonadotrophin release, 2) causes an accumulation of secretory granules, and 3) is important for maintaining secretory capacity of gonadotrophs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00217892
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  • 4
    Electronic Resource
    Electronic Resource
    Ultrastructural observation of paracrystalline aggregates of microtubules in anterior pituitary cells of the chinchilla (Chinchilla laniger) (1980)
    Springer
    Cell & tissue research 213 (1980), S. 433-440 
    Details
    ISSN: 1432-0878
    Keywords: Anterior pituitary ; Microtubules ; Paracrystalline aggregates ; Ultrastructure ; Chinchilla
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Unusual paracrystalline aggregates of microtubules which have not been described in any other mammalian species were observed in cultured anterior pituitary cells of normal chinchillas as well as in situ in the pituitary glands of these animals. These aggregates appeared as regularly arranged tubular structures in the longitudinal plane, and as a checkerboard pattern of closely and regularly packed microtubules when examined in transverse section. Supplementation with vinblastine, colcemide or colchicine in the culture medium did not change these structures morphologically. Each unit of tubules consisted of an outer wall or parellelogram profile and an inner wall composed of a single hexagonal doublet or in a figure “8” form. The outer wall of the parallelogram was 35×28 nm in length for both sides, while the diagonal of the inner wall was 18×28 nm. These paracrystalline aggregates of microtubules in the chinchilla pituitary cells are morphologically distinct from the paracrystalline assembly of cytoplasmic microtubules induced by vinblastine or other alkaloids. The function and significance of these paracrystalline aggregates in anterior pituitary cells of the chinchilla are uncertain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00237889
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