ISSN:
0739-4462
Schlagwort(e):
juvenile hormone esterase
;
Autographa californica nuclear polyhedrosis virus
;
Heliothis virescens
;
Chemistry
;
Food Science, Agricultural, Medicinal and Pharmaceutical Chemistry
Quelle:
Wiley InterScience Backfile Collection 1832-2000
Thema:
Biologie
Notizen:
Baculovirus insecticides are receiving renewed attention as insect pest control agents following the development of fast-acting recombinant baculoviruses. Here we report on the construction and biological activity of a recombinant baculovirus derived from the nuclear polyhedrosis virus of Autographa californica which expresses a modified form of juvenile hormone esterase (JHE). The serine at the catalytic site of the JHE has been mutated to a glycine residue so that the protein does not degrade JH. The recombinant baculovirus expressing this modified form of JHE, named AcJHE-SG, has enhanced activity against lepidopteran larvae. Lethal times of the recombinant are 20 to 30% lower than for the wild type virus, and a 66% reduction in feeding damage caused by infected larvae is observed. This result is comparable to the best recombinant baculovirus developed to date, AcAaIT, which expresses an insect-selective scorpion toxin. The potential of these recombinant viruses for commercialization as insecticides is discussed. Bioassays of AcJHE-SG in conjunction with anti-JH agents indicate that the virus is not killing by an anti-JH mechanism. Larvae apparently die from contraction-paralysis, or disruption of the normal sequence of events at the molt. © 1995 Wiley-Liss, Inc.
Zusätzliches Material:
2 Ill.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1002/arch.940300208
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