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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Plant Science 69 (1990), S. 111-122 
    ISSN: 0168-9452
    Keywords: 2D electrophoresis of proteins ; Helianthus annuus ; differential hybridization ; floral cDNA library
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 244 (1994), S. 312-317 
    ISSN: 1617-4623
    Keywords: Helianthus annuus ; cDNA ; Gene Signal peptide ; Conserved splice sites
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We describe here the nucleotide sequence of an anther-specific gene (sf18) from sunflower, encoding a proline- and glycine-rich polypeptide with a hydrophobic amino-terminus (signal peptide). The gene is split by a 211 by intron and is partially related to another anther-specific gene (sf2) from sunflower with which it shares important sequence stretches in the 5′ coding and upstream regions. We propose that the two genes have originated via exon shuffling, during which a copy of a DNA segment including the promoter region as well as a signal peptide coding sequence has been transferred into the upstream region of two different potential coding sequences, generating two novel genes which display the same specificity of expression and which both encode an extracellular protein. While the 5′ region of the intron is highly conserved as part of the transferred region and may play a role in the selection of the 5′ splice site, a common octanucleotide at the 3′ end of the intron of the two genes might be involved in 3′ splice site selection.
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  • 3
    ISSN: 1617-4623
    Keywords: Helianthus annuus ; DNA sequence ; Multicopy gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have isolated and sequenced an anther-specific gene from sunflower which encodes an 800-nucleotide transcript detectable in the peripheral anther cells. It contains an intron of 2615 bp, which separates the first exon (77 bp) coding for a putative signal peptide of 21 amino acids, from the second exon (563 bp) coding for a 100 amino acid polypeptide. The 5′ and 3′ untranslated regions comprise respectively 13 and 264 bp. The SF2 gene is present in the sunflower genome in several copies, all or most of which contain a closely related intron.
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  • 4
    ISSN: 1573-5028
    Keywords: Arabidopsis thaliana ; cDNA sequence ; Helianthus annuus ; immunolocalization ; RT-PCR ; zinc finger
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract LIM proteins are important eucaryotic developmental regulators characterized by the presence of one or several double zinc finger motifs, the LIM domains, which are protein-interacting domains. Using the cDNA of the previously described pollen LIM protein PLIM1 from sunflower as a hybridization probe we have isolated the coding sequence for a related protein from cDNA libraries from various sunflower organs. This protein, WLIM1, is 188 amino acids long and, like the pollen protein PLIM1, contains two LIM domains, separated by a 48 residue spacer region. The two sunflower proteins are structurally related to the animal LIM proteins CRP and MLP. A WLIM1 gene transcript was detected by RT-PCR in all vegetative and reproductive plant organs tested. Polyclonal antibodies raised against the bacterially expressed and affinity-purified protein recognize a polypeptide of ca. 50 kDa in these organs. Immunocytochemical studies detect the protein in many cell types in each of these organs where it is localized either to the cytoplasm, the nucleus, or both. The protein is often associated with plastids and smaller cellular structures or organelles. In late anaphase and early telophase of dividing cells from ovaries, stems and roots it accumulates in the phragmoplast, and may therefore also play a role in cytokinesis.
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  • 5
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 96 (1963), S. 498-508 
    ISSN: 0009-2940
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Die durch Benzoophenon photosensibilisierte Cycloaddition von Dimethyl-maleinsäureanhydrid an Thiophen und sieben Olefine verschiedener Typen führt glatt zu Cyclobutanderivaten. Das Trichloräthylen- Dimethylmaleinsäureanhydrid-Addukt geht durch HCl-Abspaltung in ein Cyclobutenderivat über, dessen Isomerisierung α.α′-Dimethyl-β.β′- dichlor-muconsäure liefert.
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  • 6
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 96 (1963), S. 520-525 
    ISSN: 0009-2940
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Dehydronorcampher (I) wird photochemisch quantitativ nach Art einer Retrodienspaltung in Cyclopendadien und Keten zerlegt. endo-Dicyclopentadien (IX) zeigt keine photochemische Retrodienspaltung. IX wird nach dem Prinzip der durch Carbonylverbindungen photosensibilisierten Cycloaddition durch intramolekulare Vierringsynthese isomerisiert. Die rein biradikalischen Reaktions- mechanismen beider Photoreaktionen werden diskutiert.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 100 (1967), S. 217-222 
    ISSN: 0009-2940
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Vinylencarbonat 1 liefert bei Belichtung in Gegenwart von Sensibilisatoren die zwei stereo-isomeren C4-Cyclodimeren 2 und 3. Das Produktverhältnis ist unabhängig vom Sensibilisator, jedoch abhängig vom Lösungsmittel. Durch alkalische Verseifung von 2 und 3 lassen sich die entsprechenden Tetrahydroxycyclobutane darstellen.
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  • 8
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 98 (1965), S. 666-684 
    ISSN: 0009-2940
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: 1-Methyl- und 1-Benzyl-2.3-dihydroxy-naphthalin werden von Kalium-nitroso-disulfonat zu roten 3-Hydroxy-naphthochinonen-(1.2) (Ib, c) oxydiert, die basenkatalysiert in farblose Dimere (IIb, c) übergehen. Unsubstituiertes 2.3-Dihydroxy-naphthalin ergibt nur ein solches Dimeres (IIa) sowie ein entsprechendes Hydrat. Beim Erwärmen mit polaren Lösungsmitteln werden die roten, monomeren Chinone zurückgebildet. Mit o-Phenylendiamin liefern die dimeren Chinone die gleichen Phenazine wie die monomeren. Den Dimeren wird eine symmetrische, durch Aldolreaktion gebildete Tricyclo[5.3.1.12.6]dodecan-Struktur zugeschrieben.  -  Durch Disproportionierung von IIa entsteht außer dem violetten, ebenfalls dimerisierbaren Chinon Id eine ockerfarbene Verbindung C20H10O7, die dem angeblichen 3-Hydroxy-naphthochinon-(1.2) der Literatur entspricht. Die bekannten, dimeren 3-Hydroxy-benzochinone-(1.2) werden analog II formuliert.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 98 (1965), S. 3854-3873 
    ISSN: 0009-2940
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Die durch Benzophenon photosensibilisierte Cycloaddition von Maleinsäureanhydrid (1) an Chlor- oder Bromolefine macht halogenierte Cyclobutanderivate leicht zugänglich. Die Mischpolymerisation mit 1 wird bereits durch ein Halogenatom an der Doppelbindung inhibiert. Aus 1 in cis-Dichloräthylen entstehen unter Erhaltung der Konfiguration die stereoisomeren cis-Dichlorcyclobutanderivate vom syn-Typ 14a und anti-Typ 15a im Verhältnis 81 : 19. Die sterische Selektion dieser Cycloaddition ist stark lösungsmittelabhängig. So dirigiert Acetonitril (hohe DK) vollständig zu 14a, während Tetrachlorkohlenstoff (niedrige DK) 15a zum Hauptprodukt macht. Beim Belichten von 1 in trans-Dichloräthylen entstehen die Cycloaddukte des cis-Dichloräthylens, das durch sensibilisierte Photoisomerisierung aus trans-Dichloräthylen hervorgeht. Die NMR-Spektren einiger ausgewählter Cyclobutanderivate zeigen eine Abhängigkeit des Vorzeichens der über vier Bindungen erfolgenden Kopplung der diagonal angeordneten Protonen von ihrer räumlichen Stellung zueinander.
    Additional Material: 3 Ill.
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  • 10
    ISSN: 0009-2940
    Keywords: Glyceraldehyde ; Ligands, tridentate ; Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A series of seven chiral, tripodal N,O,S and N,N,O ligands were prepared in which N stands for a secondary amine or imidazole donor, O for a phenol, and S for a thioether or thiol metal-binding group. Key steps are (1) the construction of ortho-hydroxyacetophenones bearing the phenolic binding group and either a thioether, a protected thiol or an imidazole substituent in the α-position, and (2) subsequent reductive amination with a primary amine. The modular synthesis allows a rapid construction of a variety of structurally related ligands. In three cases, the enantiomers of the racemic products could be separated after condensation with (R)-glyceraldehyde acetonide as chiral auxiliary. The relative configurations of the cyclic N,O- and N,N-acetals thus obtained were established by NOE spectroscopy. X-ray structural analysis of two crystalline N,O- and N,N-acetals allowed the assignment of absolute configurations. Hydrolysis of the dia-stereomerically pure acetals afforded the enantiomerically pure ligands in high yield. By comparison of their CD spectra, absolute configuration could also be assigned to the third pair of enantiomerically pure ligands.
    Additional Material: 3 Ill.
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