ISSN:
1052-9306
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
A selected ion monitoring stable isotope dilution gas chromatographic/negative ion chemical ionization mass spectrometric assay for amphetamine in human plasma is described. The preparation of (2H5)amphetamine from the intact target molecule and its use as an internal standard for quantitative gas chromatography/mass spectrometry are demonstrated. Derivatization to the highly electrophoric pentafluorobenzoate with pentafluorobenzoyl chloride in hexane resulted in high yield of prominent negative ions with methane as a moderating gas. The pentafluorobenzoate was also found to be superior to fluoroacyl derivatives with respect to sample handling due to its lower volatility. The method presented permits detection of amphetamine in the low picogram per millilitre range and should thus be highly useful for its trace level determination in forensic, toxicological and pharmaceutical applications.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bms.1200231006
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