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  • 1
    Electronic Resource
    Electronic Resource
    Conformation and interactions of the packaged double-stranded DNA genome of bacteriophage T7 (1998)
    New York, NY [u.a.] : Wiley-Blackwell
    Biospectroscopy 4 (1998), S. S47 
    Details
    ISSN: 1075-4261
    Keywords: nucleic acid ; conformation ; Raman spectroscopy ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The structure of the packaged double-stranded DNA genome of bacteriophage T7 was compared to that of unpackaged T7 DNA using digital difference Raman spectroscopy. Spectral data were obtained at 25°C from native T7 virus (100 mg/mL), empty T7 capsids (50 mg/mL), and purified T7 DNA (40 mg/mL) in buffer containing 200 mM NaCl, 10 mM MgCl2, and 10 mM Tris at pH 7.5. At these conditions, the local conformation of T7 DNA was not affected by packaging. Specifically, the local B-form secondary structure of unpackaged T7 DNA, including furanose C2′-endo pucker, anti glycosyl torsion, Watson-Crick base pairing, and base stacking, were essentially fully (〉98%) retained when the genome was condensed within the viral capsid. However, the average electrostatic environment of T7 DNA phosphates was altered dramatically by packaging as revealed by large perturbations in the Raman bands associated with localized vibrations of the DNA phosphate groups. The change in the phosphate environment was attributed to Mg2+ ions that were packaged with the genomic DNA, and the observed Raman perturbations of genomic DNA were equivalent to those generated by a 50-100-fold increase in Mg2+ concentration in aqueous phosphodiester model compounds. The T7 data were qualitatively and quantitatively similar to those observed previously for packaged DNA of bacteriophage P22 and imply that genomic DNAs of T7 and P22 are both organized in a similar fashion within their respective capsids. The results show that the condensed genome does not contain kinks or folds that would disrupt the local B conformation by more than 2%. The present findings are discussed in relation to previously proposed models for condensation and organization of double-stranded and single-stranded viral DNA. © 1998 John Wiley & Sons, Inc. Biospectroscopy 4: S47-S56, 1998
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
  • 2
    Electronic Resource
    Electronic Resource
    Ion-spray mass spectrometry of marine neurotoxinsNRCC 29950. (1989)
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 3 (1989), S. 145-150 
    Details
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: Ion-spray mass spectrometry was investigated for the analysis of three marine neurotoxins: domoic acid, saxitoxin and tetrodotoxin. All three compounds gave positive-ion spectra with abundant ions of protonated molecules and no significant fragmentation. Domoic acid gave a negative-ion spectrum with a strong [M-H]-ion. Tandem mass spectrometry provided useful fragment-ion spectra for all compounds. Detection limits for flow injection analyses with selected-ion monitoring were determined to be 30 pg for saxitoxin, 100 pg for domoic acid and 200 pg for tetrodotoxin. Combining liquid chromatography with ion-spray mass spectrometry allowed the determination of domoic acid and some of its isomers in toxic shellfish tissue extracts.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/rcm.1290030508
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  • 3
    Electronic Resource
    Electronic Resource
    Polyethylene Glycol: A reagent for tuning and calibrating mass spectrometers for positive-ion atmospheric pressure chemical ionization (1995)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 30 (1995), S. 381-383 
    Details
    ISSN: 1076-5174
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jms.1190300222
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  • 4
    Electronic Resource
    Electronic Resource
    Modern methods for trace element determination. C. Vandercasteele and C. B. Block Wiley, Chichester, 1993, 330pp., ISBN 0471940399 (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    X-Ray Spectrometry 24 (1995), S. 38-38 
    Details
    ISSN: 0049-8246
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/xrs.1300240108
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  • 5
    Electronic Resource
    Electronic Resource
    Photodissociation of chlorostyrene molecular ions (1986)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 21 (1986), S. 447-448 
    Details
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/oms.1210210716
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  • 6
    Electronic Resource
    Electronic Resource
    1H and 13C NMR assignments for lanostan-3β-ol derivatives: Revised assignments for lanosterol (1989)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 27 (1989), S. 1012-1024 
    Details
    ISSN: 0749-1581
    Keywords: 1H and 13C NMR ; Oxygenated lanostane derivatives ; Spectral assignment ; Lanthanide-induced shifts ; Long-range HETCOR ; Acetylation shifts ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: 1H and 13C NMR assignments are presented for 30 oxygenated lanostane derivatives, including lanosterol, dihy-drolanosterol, 7-ketolanosterol, agnosterol, 24,25-epoxylanosterol, 8α,9α-epoxylanostan-3β-ol, three 15-oxygenated derivatives of lanost-7-en-3β-ol, lanostane-3β,7α-diol, lanostane-3β,9α-diol and their acetates. These assignments, which were largely determined by a combination of DEPT, one-boad and long-range 13C—1H chemical shift correlation and lanthanide-induced shift experiments, are not dependent on previously reported assignments, several of which were found to be incorrect. 1H and 13C acetylation shifts for lanostan-3β-ols were sufficiently invariant among the sterols studied that they were useful for assigning carbons in rings A and B. The acetylation shifts reported for lanostan-3β-ols were extended and partially revised.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrc.1260271103
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  • 7
    Electronic Resource
    Electronic Resource
    Secondary structure of the double-stranded DNA genome of bacteriophage T7 in packaged, underpackaged and unpackaged statesPart XXVIII in the series Studies of Virus Structure by Raman Spectroscopy. (1990)
    Chichester [u.a.] : Wiley-Blackwell
    Journal of Raman Spectroscopy 21 (1990), S. 569-575 
    Details
    ISSN: 0377-0486
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Double-stranded DNA exhibits prominent Raman bands in the spectral region 600-900 cm-1, authenticated by x-ray crystallography as diagnostic of the macromolecular secondary structure. Since the DNA Raman fingerprint can be measured in the presence of protein, Raman spectroscopy was employed to investigate the secondary structure of dsDNA packaged within the protein coat of bacteriophage T7. Comparison of the Raman spectrum of deproteinized DNA with that of wild-type T7 phage (52% DNA, 48% protein) reveals a packaging-associated shift of the principal B—DNA Raman marker (the band of the 825-840 cm-1 interval which is a sensitive indicator of phosphodiester geometry), without shifts of other conformation sensitive Raman markers (the bands of the 600-780 cm-1 interval which indicate sugar puckers and glycosyl torsions). The Raman marker for the protein-free state occurs at 836 cm-1, typical of B-form DNA of heterogeneous sequence; the marker for the packaged state occurs at 825 cm-1, characteristic of a modified B-form DNA observed previously for specific sequences. These findings indicate different B-form variants for dsDNA in packaged and protein-free states. The modified B-form secondary structure of packaged DNA is maintained when the virion is thermally burst to release the genome without removal of proteins.Comparison of the Raman spectrum of the wild-type T7 virion with that of a deletion mutant packaging 8.4% less DNA than wild-type (underpackaged T7), reveals the same packaging-associated shift of the principal B-form marker (825 cm-1) vis-à-vis protein-free DNA (836 cm-1), and additional evidence of small but significant differences between phosphodiester geometries in packaged and underpackaged states. These differences are explained by a marginally (〈5%) greater proportion of non-B backbone geometry in wild-type T7. Both wild-type and mutant T7 exhibit Raman markers of the C2′-endo/anti nucleoside conformations exclusively. The results are interpreted in terms of the formation of increasing amounts of non B-form phosphodiester geometry with increasing density of DNA packaging. The data are consistent with an excess of 〈5% A-type phosphodiester geometry in packaged versus underpackaged states, and 10 ± 4% excess A-type phosphodiester geometry in packaged versus protein-free states. Based on Raman amide I and amide III markers, the protein structures and protein-DNA interactions in wild-type T7 are indistinguishable from those in the T7 deletion mutant.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jrs.1250210908
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