ISSN:
0749-1581
Keywords:
Brassinosteroids
;
13C NMR
;
2D NMR
;
Brassinolide
;
Steroids
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
1H NMR spectra of eight naturally occurring brassinosteroids, plant hormones with a steroid skeleton, and five synthetic analogues were analysed by COSY and long-range COSY measurements. Based on the 1H resonances, the 13C signals were assigned by a combination of C—H COSY and long-range C—H COSY spectra detecting 13C nuclei, or by HMQC and HMBC spectra detecting 1H nuclei. Although the steroids showed complex 1H signals around 1-2 ppm, the resonances for the two angular methyl groups and the other methyl groups in the side-chain could be assigned unambiguously and used as a reference point for the other assignments. Strong correlation peaks between the methyl protons and carbons connected with the protons through two or three bonds were observed in the long-range C—H COSY or the HMBC spectra. These spectra provide valuable information for assigning the 13C NMR resonances of this class of compounds.
Additional Material:
1 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/mrc.1260310119
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