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  • Analytical Chemistry and Spectroscopy  (2)
  • Free flow electrophoresis  (2)
  • 1
    Electronic Resource
    Electronic Resource
    On-line isotachophoretic sample preconcentration for enhancement of zone detectability in capillary zone electrophoresis (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Microcolumn Separations 2 (1990), S. 229-233 
    Details
    ISSN: 1040-7685
    Keywords: capillary isotachophoresis ; capillary zone electrophoresis ; detectability enhancement ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A simple isotachophoretic (ITP) preconcentration column for subsequent online capillary zone electrophoresis is described, and its use for enhancing detectability in capillary zone electrophoresis (CZE) is evaluated. Sample preconcentration using ITP is shown to enhance detectability in CZE by several orders of magnitude. Experimental verification of the detectability gain is demonstrated for cationic and anionic separations; an increase in detectability of more than 200-fold was achieved. The practical applicability of this method is demonstrated using a direct analysis of thiamine in human blood.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mcs.1220020505
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  • 2
    Electronic Resource
    Electronic Resource
    Determination of pyruvate, acetoacetate, lactate, and 3-hydroxybutyrate in plasma of patients with diabetes mellitus by capillary isotachophoresis (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Microcolumn Separations 2 (1990), S. 80-83 
    Details
    ISSN: 1040-7685
    Keywords: capillary isotachophoresis ; determination of pyruvate ; acetoacetate ; 3-hydroxybutyrate ; lactate ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: An isotachophoretic method was developed and optimized for simultaneous determination of pyruvate, acetoacetate, lactate, and 3-hydroxybutyrate in 1-10 μL of untreated heparin plasma of patients with diabetes mellitus. A commercial instrument with a column switching system was used. The preseparation capillary was filled with the leading electrolyte of pH 4.2 and the analytical capillary was filled with leading electrolyte of pH 3.0. The concentrations of individual ketone bodies and lactate found in human plasma varied within 0.01-1.4 mmol/L and 0.8-11.8 mmol/L, respectively. Analysis time was about 30 min.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mcs.1220020207
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  • 3
    Electronic Resource
    Electronic Resource
    Optimized continuous flow electrophoresis (1996)
    Weinheim : Wiley-Blackwell
    Electrophoresis 17 (1996), S. 1906-1910 
    Details
    ISSN: 0173-0835
    Keywords: Continuous flow electrophoresis ; Free flow electrophoresis ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Continuous flow electrophoresis (CFE) was optimized by employing (i) electrophoretic regimes with stacking properties, to eliminate electrohydrodynamic dispersion, (ii) quasi-mixed zones to prevent precipitation of the stacked analytes, (iii) sheath liquid streams at the electrode compartment membranes to prevent penetration of the electrode reaction products into the separation chamber, (iv) proper engineering of the separation chamber to provide efficient dissipation of Joule heat, and (v) counterflow at the collection outlets to eliminate the problems of dead volumes and uneven collection of separated species. Data on direct temperature measurements in the separation chamber at various levels of the dissipated electric power are presented. Preparative runs of amyloglucosidase in the isoelectric focusing (IEF) mode and rat liver organelles in the isotochophoresis (ITP) mode demonstrate the high performance of the optimized CFE system.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150171216
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  • 4
    Electronic Resource
    Electronic Resource
    Recent developments in preparative free flow isoelectric focusing (1998)
    Weinheim : Wiley-Blackwell
    Electrophoresis 19 (1998), S. 1649-1653 
    Details
    ISSN: 0173-0835
    Keywords: Free flow electrophoresis ; Preparative electrophoresis ; Continuous isoelectric focusing ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Continuous flow electrophoresis (CFE) is a promising method for preparative fractionation of a variety of biological species, ranging from peptides and proteins to subcellular particles and cells. The high separation efficiency of FFE may be deteriorated by hydrodynamic distortion of zones due to the omnipresent parabolic laminar flow profile. We show in this paper that the detrimental hydrodynamic distortion of separated proteins zones can be reduced, with resultant enhancement of separation efficiency, by employing continuous isoelectric focusing in pH gradients as the actual working regime in an advanced instrumentation. Newly developed media for fast generation of narrow- or broad-range pH gradients under CFE conditions are described. The separation efficiency of these pH gradients is comparable to that of the gradients formed with the aid of synthetic carrier ampholytes. The new media are defined mixtures of nontoxic chemicals, and thus they are compatible with the requirements of human medicine. Experimental data are given showing that the new media offer fractionation of isoforms of proteins, that they offer resolution of proteins differing in isoelectric point (pI) by less than 0.05 pH units, and that these media inhibit proteins precipitation in experiments with human serum proteins.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150191021
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