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  • 1
    Electronic Resource
    Electronic Resource
    Cellodextrin utilization and β-glucosidase production by Bacteroides polypragmatus (1986)
    Springer
    Archives of microbiology 145 (1986), S. 91-96 
    Details
    ISSN: 1432-072X
    Keywords: Cellobiose ; Cellodextrins ; Anaerobic ; Ethanol ; β-Glucosidase ; Bacteroides polypragmatus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Bacteroides polypragmatus, a mesophilic obligate anaerobe, was shown to simultaneously ferment glucose and cellobiose giving ethanol as a major metabolic end-product. A mixture of higher cellodextrins was also utilized. The bacterium produced a β-glucosidase with a pI value of 4.2 and a molecular weight of approximately 100000 daltons. The enzyme was intracellular and functioned optimally at pH 7. The K m values obtained with p-nitrophenyl-β-d-glucoside and cellobiose as substrates were 0.73 mM and 100 mM, respectively. The enzyme was quite stable at elevated temperatures; in the presence of 10% glycerol (v/v), it had a half-life of 4 h at 55°C. It was also stable during long-term storage at either 4°C or-20°C, provided that 10% (v/v) glycerol was added to preparations maintained at-20°C.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00413033
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  • 2
    Electronic Resource
    Electronic Resource
    Growth and butyric acid production by Clostridium populeti (1988)
    Springer
    Archives of microbiology 150 (1988), S. 267-271 
    Details
    ISSN: 1432-072X
    Keywords: Clostridium ; Butyric acid ; Nutrition ; Glucose ; Fermentation ; Anaerobe
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The growth of Clostridium populeti in 2% (w/v) glucose medium containing 0.2% (w/v) yeast extract was optimal with 10 mM NH4Cl as the nitrogen source. Although the maximum specific growth rate (μ=0.32 h-1) with 5 mM NH4Cl was similar, the biomass yield was about 30% lower than that at the optimum. Either sodium sulphide or cysteine-HCl at an optimum concentration of 0.33 mM and 5.0 mM respectively, could serve as the sole sulphur source for growth. The growth rate was unaffected by initial glucose concentrations of up to 10% (w/v), but in the presence of 15% glucose it declined by about 35%. The molar yield of butyric acid (mol/mol glucose) declined from 0.70 in 1% (w/v) initial glucose medium to 0.39 in 10% glucose medium. In 5.7% initial glucose medium, butyric acid levels of 6.3 g/l were obtained (0.56 mol butyrate/mol glucose) after 72 h of incubation in 2.5 l batch cultures. A decrease of about 50% in the maximum specific growth rate of C. populeti was observed in the presence of an initial concentration of either 1.2 g/l of butyric acid or 18.9 g/l of acetic acid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00407790
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