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  • Electron irradiation damage  (2)
  • Amorphous  (1)
  • DNA disruption  (1)
  • 1
    ISSN: 1617-4623
    Keywords: dnaKmutant ; Heat shock protein ; Superoxide dismutase ; DNA disruption ; Escherichia coli K12
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary IndnaK7(Ts) mutant cells, scission of DNA strands occurred after temperature shift up. When cells at 30°C were labeled with [3H]-thymidine and then shifted to 46° or 49°C for 20 min, the profiles of sedimentation of thier cellular DNA in an alkaline sucrose gradient revealed a decrease in the size of DNA to a quarter of that at 30°C in the mutant, but not in wild-type cells. The level of manganese-containing superoxide dismutase (MnSOD) in the mutant was about twice that in wild-type cells, even at the permissive temperature, implying increased production of superoxide radical anion, which may cleave DNA strands directly or indirectly in the mutant. Moderate increase in the MnSOD level on temperature shift up was observed in both strains. These results indicated that some components of the DnaK protein participate in protection of cellular membrane functions from thermal damage resulting from elevated production of the superoxide anion radical.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 3 (1986), S. 45-56 
    ISSN: 0741-0581
    Keywords: High voltage electron microscopy ; High resolution electron microscopy ; Amorphous ; Amorphization ; Niti alloy ; Mosaic block ; Electron irradiation damage ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Amorphization induced by electron irradiation in Ni-50 at% Ti crystals has been investigated by electron microscopy. In order to clarify successive stages of amorphization, micrographs were taken in vairous regions where the total dose of electrons was continuously changed. Contrast was manipulated by changing the reflecting condition. Results obtained are summarized as follows: (a) Amorphization occurs inhomogenously; (b) there is a critical size, i.e., about 5 nmø in the crystals, for observing sharp lattice images in small blocks; (c) lattice images change to short and wavy fringe contrasts when the block size becomes smaller than the critical one, and finally they disappear; and (d) boundary contrast of the blocks rapidly decreases with decreasing size, and is hardly observed when the size becomes smaller than the critical one. Based on the results, the cause of abnormal fringe contrasts in fine blocks, the amorphization process, and atomic structures of amorphous materials are discussed.
    Additional Material: 7 Ill.
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 12 (1989), S. 201-218 
    ISSN: 0741-0581
    Keywords: Very-high-temperature stage ; Electron irradiation damage ; In situ deformation ; Ductile ceramics ; Amorphization ; Foreign atom implantation ; Environment - material interaction ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: High-voltage electron microscopy has shown itself advantageous for the study of natural science, including biology, but especially for materials science. The most important advantage for materials science is for in situ experiments about the detailed processes of the phenomena that occur in bulk materials. The present paper is mainly concerned with several types of in situ experiments that have been carried out in the Research Center for Ultra-High Voltage Electron Microscopy, Osaka University. The following subjects have been studied: (a) fundamental problems, such as the conditions necessary for in situ experiments, functional features of specimen treatment devices, and the effects of electron irradiation; (b) the dislocation behavior of crystals under various conditions; (c) high-temperature behavior of refractory materials, mainly ceramic composites; (d) new applications of electron irradiation effects, such as amorphization of crystalline materials and electron-irradiation-induced foreign-atom implantation; (e) environment-matter interaction, mainly chemical amorphization of alloys; and (f) future trends of the in situ experiment, such as combinations with Auger valency electron spectroscopy and high-resolution electron microscopy.
    Additional Material: 18 Ill.
    Type of Medium: Electronic Resource
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