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  • LIFE SCIENCES (GENERAL)  (14)
  • Amoeba proteus  (2)
  • Induced pinocytosis  (2)
  • Life Sciences (General)  (2)
  • VAE 120  (2)
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Keywords
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  • 1
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    High-Resolution Imaging of Texture and Microstructure by the Moving Detector Method (2010)
    Details
    Publication Date: 2021-03-29
    Description: In order to describe texture and microstructure of a polycrystalline material completely, crystal orientation g={?1F?2} must be known in all points x={x1?x2?x3} of the material. This can be achieved by locationresolved diffraction of high-energy, i.e. short-wave, X-rays from synchrotron sources. Highest resolution in the orientation- as well as the location-coordinates can be achieved by three variants of a detector sweeping technique in which an area detector is continuously moved during exposure. This technique results in two-dimensionally continuous images which are sections and projections of the six-dimensional orientation location space. Further evaluation of these images depends on whether individual grains are resolved in them or not. Because of the high penetration depth of high-energy synchrotron radiation in matter, this technique is also, and particularly, suitable for the investigation of the interior of big samples.
    Description: research
    Keywords: 548 ; VAE 120 ; VKA 200 ; VGA 410 ; Methodik {Strukturgeologie} ; Gefügekunde der Gesteine ; Röntgenanalyse {Mineralogie: Kristallographie}
    Language: English
    Type: article , publishedVersion
    Format: 18 S.
    Format: application/pdf
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    CITATION GEO-LEO
  • 2
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    High-Resolution Imaging of Texture and Microstructure by the Moving Detector Method (2018)
    Details
    Publication Date: 2021-03-29
    Description: In order to describe texture and microstructure of a polycrystalline material completely, crystal orientation g={?1F?2} must be known in all points x={x1?x2?x3} of the material. This can be achieved by locationresolved diffraction of high-energy, i.e. short-wave, X-rays from synchrotron sources. Highest resolution in the orientation- as well as the location-coordinates can be achieved by three variants of a detector sweeping technique in which an area detector is continuously moved during exposure. This technique results in two-dimensionally continuous images which are sections and projections of the six-dimensional orientation location space. Further evaluation of these images depends on whether individual grains are resolved in them or not. Because of the high penetration depth of high-energy synchrotron radiation in matter, this technique is also, and particularly, suitable for the investigation of the interior of big samples.
    Keywords: 551 ; VAE 120 ; VKA 200 ; VGA 410 ; 38.03
    Language: English
    Type: article , publishedVersion
    Format: application/pdf
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    CITATION GEO-LEO
  • 3
    Electronic Resource
    Electronic Resource
    Pinocytosis and locomotion in amoebae (1979)
    Springer
    Protoplasma 100 (1979), S. 33-43 
    Details
    ISSN: 1615-6102
    Keywords: Amoeba proteus ; Ca++-binding sites ; Cytochemical demonstration ; Induced pinocytosis ; Plasma membrane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Different methods were used to demonstrate the existence of Ca++-binding sites (Ca++-bs) at the plasma membrane ofAmoeba proteus. In pinocytoting animals the number (indicated by the average distanced in nm) and size (average longitudinal axiss in nm) of Ca++-bs at the cytoplasmic surface of the cell membrane were significantly increased (d=162±15;n=41 ands=93±5;n=47) in comparison to controls (d=208 ±21;n=37 ands=59±8;n=45). The ratio of P: Ca obtained by X-ray microanalysis was in the range of 1.5. The differences observed in the two experimental groups of amoebae are explained by conformational changes in the molecular structure and an increased Ca++-permeability of the plasma membrane during induced pinocytosis. Microplasmodia of the acellular slime moldPhysarum polycephalum investigated for comparison were found to have no Ca++-bs at the interior cell surface.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01276299
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Pinocytosis and locomotion of amoebae: XII. Dynamics and motive force generation during induced pinocytosis in A. proteus (1979)
    Springer
    Cell & tissue research 197 (1979), S. 263-279 
    Details
    ISSN: 1432-0878
    Keywords: Induced pinocytosis ; Dynamics ; Motive force generation ; Light and electron microscopy ; Amoeba proteus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The mechanism of induced pinocytosis was investigated in Amoeba proteus by light and electron microscopy. The application of nine different inducing substances revealed that pinocytotic channel formation, elongation, vesiculation, shortening and disappearance are the result of the successive or simultaneous action of both traction and pressure forces, which are produced by the contractile activity of a plasma membrane-associated layer of filaments ranging from a few hundred nm to several μ in thickness. The initial phase of channel formation is caused by traction forces according to the membrane flow concept, whereas channel elongation and vesiculation mainly result from pressure forces in conjunction with the extrusion of small hyaline pseudopodia. Shortening and disappearance of the pinocytotic channels are brought about by local contractions of the cortical filament layer in the basal region of the hyaline pseudopodia. Experiments using latex beads as marker particles together with inducing substances show that a rapid membrane turnover during pinocytosis can be excluded, and that the plasma membrane slides as an entire structure over the underlying cytoplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233919
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    Paper (German National Licenses)
    Fulltext
  • 5
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    Studies of specific hepatic enzymes involved in the conversion of carbohydrates to lipids in rats exposed to prolonged spaceflight aboard Cosmos 1129 (1982)
    In:  Other Sources
    Details
    Publication Date: 2011-08-18
    Description: (Previously announced in STAR as N81-32831)
    Keywords: LIFE SCIENCES (GENERAL)
    Format: text
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    NASA TECHNICAL REPORTS
  • 6
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    Stabilization of the yeast desaturase system by low levels of oxygen (1983)
    In:  Other Sources
    Details
    Publication Date: 2011-08-18
    Description: The stability of particulate palmitoyl-CoA desaturase preparations from anaerobically grown yeast cells was increased by exposure to low levels of oxygen. The stabilizing effect of oxygen may be based upon the increased amounts of palmitoleic acid and ergosterol that become available to the cells. These results suggest the evolutinary appearance of this system at a time when atmospheric oxygen was at a low level.
    Keywords: LIFE SCIENCES (GENERAL)
    Type: Origins of Life (ISSN 0302-1688); 13; March 19
    Format: text
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    NASA TECHNICAL REPORTS
  • 7
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    Factors affecting the palmitoyl-coenzyme A desaturase of Saccharomyces cerevisiae (1975)
    In:  Other Sources
    Details
    Publication Date: 2011-08-16
    Description: The activity and stability of the palmitoyl-coenzyme A (CoA) desaturase complex of Saccharomyces cerevisiae was influenced by several factors. Cells, grown nonaerobically and then incubated with glucose, either in air or under N2, showed a marked increase in desaturase activity. Cycloheximide, added during such incubations, prevented the increase in activity, suggesting de novo synthesis. The stability of the desaturase from cells grown nonaerobically was affected by subsequent treatment of the cells; enzyme from freshly harvested cells, or from cells that were then shaken under nitrogen, readily lost activity upon washing or during density gradient analysis, whereas aerated cells, in the presence or absence of glucose, yielded stable enzyme preparations. The loss of activity in nonaerobic preparations could be reversed by adding soluble supernatant from these homogenates and could be prevented by growing the cells in the presence of palmitoleic acid and ergosterol, but not with several other lipids tested.
    Keywords: LIFE SCIENCES (GENERAL)
    Type: Journal of Bacteriology; 124; Nov. 197
    Format: text
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    NASA TECHNICAL REPORTS
  • 8
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    Oxygen as a factor in eukaryote evolution - Some effects of low levels of oxygen on Saccharomyces cerevisiae (1979)
    In:  Other Sources
    Details
    Publication Date: 2011-08-17
    Description: A comparative study of the effects of varying levels of oxygen on some of the metabolic functions of the primitive eukaryote, Saccharomyces cerevisiae, has shown that these cells are responsive to very low levels of oxygen: the level of palmitoyl-Co A desaturase was greatly enhanced by only 0.03 vol % oxygen. Similarly, an acetyl-CoA synthetase associated predominantly with anaerobic growth was stimulated by as little as 0.1% oxygen, while an isoenzyme correlated with aerobic growth was maximally active at much higher oxygen levels (greater than 1%). Closely following this latter pattern were three mitochondrial enzymes that attained maximal activity only under atmospheric levels of oxygen.
    Keywords: LIFE SCIENCES (GENERAL)
    Type: Origins of Life; 9; Sept
    Format: text
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    NASA TECHNICAL REPORTS
  • 9
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    Subunit specificity of the two acetyl-CoA synthetases of yeast as revealed by an immunological approach (1980)
    In:  Other Sources
    Details
    Publication Date: 2011-08-18
    Keywords: LIFE SCIENCES (GENERAL)
    Type: Biochimica et Biophysica Acta; 614; 1980
    Format: text
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    NASA TECHNICAL REPORTS
  • 10
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    Experiment K304: Studies of specific hepatic enzymes and liver constituents involved in the conversion of carbohydrates to lipids in rats exposed to prolonged space flight (1981)
    In:  Other Sources
    Details
    Publication Date: 2011-08-18
    Description: The effects of space flight on the activities of 26 enzymes concerned with carbohydrate and lipid metabolism in hepatic tissue taken from male Wistar rats are investigated. These activities were measured in the various hepatic cell compartments, i.e., cytosol, mitochondria and microsomes. In addition, the levels of glycogen, total lipids, phospholipids, triglycerides, cholesterol, cholesterol esters, and the fatty acid composition of the rat livers were also examined and quantified. A similar group of ground-based rats treated in an identical manner served as controls. Both flight and synchronous control rats were sacrificed at three time intervals: R+0, 7-11 hours after recovery; R+6, after 6 days; R+6(S), after 6 days (having undergone 2-5 hour periods of fixed stress in a "backupward" position on days 0, 3, 4, 5 and 6) and R+29, after 29 days post-flight. Although most of the enzyme activities and the amounts of liver constituents studied were unaffected by the period of weightlessness, some significant differences were observed.
    Keywords: LIFE SCIENCES (GENERAL)
    Type: US Rat Expts. Flown on the Soviet Satellite Cosmos 1129; p 35-100
    Format: text
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    NASA TECHNICAL REPORTS
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