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Digitale Medien

Pinocytosis and locomotion in amoebae (1979)

Stockem, W. ; Klein, H. -P.

Springer

Protoplasma 100 (1979), S. 33-43

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ISSN: 1615-6102

Schlagwort(e): Amoeba proteus ; Ca++-binding sites ; Cytochemical demonstration ; Induced pinocytosis ; Plasma membrane

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Summary Different methods were used to demonstrate the existence of Ca++-binding sites (Ca++-bs) at the plasma membrane ofAmoeba proteus. In pinocytoting animals the number (indicated by the average distanced in nm) and size (average longitudinal axiss in nm) of Ca++-bs at the cytoplasmic surface of the cell membrane were significantly increased (d=162±15;n=41 ands=93±5;n=47) in comparison to controls (d=208 ±21;n=37 ands=59±8;n=45). The ratio of P: Ca obtained by X-ray microanalysis was in the range of 1.5. The differences observed in the two experimental groups of amoebae are explained by conformational changes in the molecular structure and an increased Ca++-permeability of the plasma membrane during induced pinocytosis. Microplasmodia of the acellular slime moldPhysarum polycephalum investigated for comparison were found to have no Ca++-bs at the interior cell surface.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF01276299

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Digitale Medien

Cation selective tubular carrier-PVC-membranes containing polymeric plasticizer with low tendency of migration for Solid State Contact Electrodes (1994)

Schindler, J. G. ; Herna, K. ; Reisinger, E. ; [weitere]

New York, NY : Wiley-Blackwell

Journal für Praktische Chemie/Chemiker-Zeitung 336 (1994), S. 575-580

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ISSN: 0941-1216

Schlagwort(e): Chemistry ; Organic Chemistry

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie

Notizen: The lifetime of cation selective carrier-PVC-membranes partially depends on the components' remaining in the membrane. An exchange of monomeric by polymeric plasticizers with low tendency to migrate lengthens the function time drastically. Other than for Na+ - and NH4+-selective membranes, it is essential for K+ - and Ca2+-selective membranes and optional for H+-selective membranes to incorporate lipophilic anions in order to make the phase transfer catalysis more efficient. The resistence to saponification of phthalic acid polyester gives H+-selective membranes a high stability of measured values even in the alkaline range.For anion selective PVC-membranes, instead of cation selective plasticizers the plasticizing qualities of a liquid charged ligand should be used.The tubular carrier-PVC-membranes of our ion selective flow through measuring systems are diffusion welded to the ends of two PVC-tubes [1] so that they are absolutely tight with no risk of potential leakage. Migration of the membrane components plasticizer and ionophore across this border as well as their extraction [2, 3] into the measuring solution [4] will naturally reduce the membrane's functionning time [5].

Zusätzliches Material: 3 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/prac.19943360703

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Digitale Medien

Pinocytosis and locomotion of amoebae: XII. Dynamics and motive force generation during induced pinocytosis in A. proteus (1979)

Klein, H. P. ; Stockem, W.

Springer

Cell & tissue research 197 (1979), S. 263-279

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ISSN: 1432-0878

Schlagwort(e): Induced pinocytosis ; Dynamics ; Motive force generation ; Light and electron microscopy ; Amoeba proteus

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Summary The mechanism of induced pinocytosis was investigated in Amoeba proteus by light and electron microscopy. The application of nine different inducing substances revealed that pinocytotic channel formation, elongation, vesiculation, shortening and disappearance are the result of the successive or simultaneous action of both traction and pressure forces, which are produced by the contractile activity of a plasma membrane-associated layer of filaments ranging from a few hundred nm to several μ in thickness. The initial phase of channel formation is caused by traction forces according to the membrane flow concept, whereas channel elongation and vesiculation mainly result from pressure forces in conjunction with the extrusion of small hyaline pseudopodia. Shortening and disappearance of the pinocytotic channels are brought about by local contractions of the cortical filament layer in the basal region of the hyaline pseudopodia. Experiments using latex beads as marker particles together with inducing substances show that a rapid membrane turnover during pinocytosis can be excluded, and that the plasma membrane slides as an entire structure over the underlying cytoplasm.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00233919

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Digitale Medien

Substituierte Alkinyle als axiale Liganden an häminähnlich gebundenem Eisen(III) - Einordnung in eine spektrochemische SerieHerrn Professor Dr. E. Hoyer zum 60. Geburtstag gewidmet (1991)

Jäger, E.-G. ; Hähnel, H. ; Klein, H.-F. ; [weitere]

New York, NY : Wiley-Blackwell

Journal für Praktische Chemie/Chemiker-Zeitung 333 (1991), S. 423-430

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ISSN: 0021-8383

Schlagwort(e): Chemistry ; Organic Chemistry

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie

Notizen: Substituted Alkinyles as Axial Ligands at Hemine Like Bound Iron(III) - Incorporation into a Spectrochemical Series.Substituted lithium alkynyles Li—C≡C—R (R = tBu, Ph, p-Cl—C6H4, Me3Si, iPr3Si, Ph3Si) react with the hemine like macrocyclic iron(III) complex 6,13-di(ethoxycarbonyl)-5, 14-dimethyl-1, 4, 8, 11-tetraazatetradeca-4,6,12,14-tetraenato[2-]iron(III)-iodide (formula 2;) in tetrahydrofuran to form anionic low-spin di-adducts [fe(C≡C—R)2]-. The incorporation of the alkynyles into a spectrochemical series of the axial ligands (studied by the sharp equatorial-ligand-to-metal CT absorption band) results in the wavelength-sequence (nm): OH- (≍ 510) « N3- (≍ 625) 〈 tBu—C≡C- (664) 〈 NH3 (666) 〈 Ph—C≡C- (692) 〈 Ph—NH2 (695) 〈 Me3Si—C≡C- (698) 〈 SCN- (713) 〈 Ph3Si—C ≡ C- (716) 〈 CN- (739) 〈 4-picoline (759) 〈 pyridine (765) 〈 nicotinamide (776) 〈 methylnicotinat (788) 〈 pyrazine (798) and points to a significant π-acceptor ability of the silyl substituents.

Zusätzliches Material: 2 Tab.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/prac.19913330307

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