ISSN:
1432-1432
Keywords:
Adenosine Cyclic 2′, 3′-Phosphate
;
Polymerization
;
1, 2-Diaminoethane
;
Oligonucleotides
;
Prebiotic
;
Gel Permeation
;
Ion Exchange
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Adenosine cyclic 2′, 3′-phosphate has been polymerized at room temperature in the dry state using 1, 2-diaminoethane at alkaline pH as catalyst. The high molecular weight products have been analyzed by several different methods, including ion exchange and gel permeation chromatography and phosphate end-group analysis. Gel permeation chromatography showed that between 3.6% and 8.1% of material higher than the hexamer was formed in 3-, 15- and 40-day reactions. The material from a large-scale, 3-day reaction mixture was first fractionated by ion exchange chromatography and the highest molecular weight material then further fractionated by gel permeation chromatography. End-group analysis on the fractions obtained by this procedure showed that 0.67% of the total reaction mixture was polymer with an average chainlength of 13.4; a trace (0.15%) of higher polymer was also formed, but there was insufficient to determine its average chainlength. The prebiological relevance of these findings is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01796557
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