Publication Date:
1991-01-25
Description:
The Bicoid homeodomain protein controls anterior development in the Drosophila embryo by binding to DNA and regulating gene expression. With the use of genetic assays in yeast, the interaction between the Bicoid homeodomain and a series of mutated DNA sites was studied. These experiments defined important features of homeodomain binding sites, identified specific amino acid-base pair contacts, and suggested a model for interaction of the recognition alpha-helices of Bicoid and Antennapedia-class homeodomain proteins with DNA. The model is in general agreement with results of crystallographic and magnetic resonance studies, but differs in important details. It is likely that genetic studies of protein-DNA interaction will continue to complement conventional structural approaches.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hanes, S D -- Brent, R -- New York, N.Y. -- Science. 1991 Jan 25;251(4992):426-30.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1671176" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Animals
;
Base Sequence
;
DNA/*metabolism
;
DNA-Binding Proteins/*genetics/metabolism
;
Drosophila
;
Gene Expression Regulation
;
Genes, Homeobox/*genetics
;
*Homeodomain Proteins
;
Insect Hormones/*genetics/metabolism
;
*Models, Genetic
;
Molecular Sequence Data
;
*Trans-Activators
;
Transcription, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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