Publication Date:
1990-09-14
Description:
A complementary DNA clone (designated GAT-1) encoding a transporter for the neurotransmitter gamma-aminobutyric acid (GABA) has been isolated from rat brain, and its functional properties have been examined in Xenopus oocytes. Oocytes injected with GAT-1 synthetic messenger RNA accumulated [3H]GABA to levels above control values. The transporter encoded by GAT-1 has a high affinity for GABA, is sodium-and chloride-dependent, and is pharmacologically similar to neuronal GABA transporters. The GAT-1 protein shares antigenic determinants with a native rat brain GABA transporter. The nucleotide sequence of GAT-1 predicts a protein of 599 amino acids with a molecular weight of 67 kilodaltons. Hydropathy analysis of the deduced protein suggests multiple transmembrane regions, a feature shared by several cloned transporters; however, database searches indicate that GAT-1 is not homologous to any previously identified proteins. Therefore, GAT-1 appears to be a member of a previously uncharacterized family of transport molecules.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Guastella, J -- Nelson, N -- Nelson, H -- Czyzyk, L -- Keynan, S -- Miedel, M C -- Davidson, N -- Lester, H A -- Kanner, B I -- GM 10991/GM/NIGMS NIH HHS/ -- GM 29836/GM/NIGMS NIH HHS/ -- NS 16708/NS/NINDS NIH HHS/ -- New York, N.Y. -- Science. 1990 Sep 14;249(4974):1303-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Division of Biology, California Institute of Technology, Pasadena 91125.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1975955" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Animals
;
Base Sequence
;
Brain/metabolism
;
Carrier Proteins/antagonists & inhibitors/*genetics/metabolism
;
Chlorine/physiology
;
Cloning, Molecular
;
GABA Plasma Membrane Transport Proteins
;
Gene Expression
;
Membrane Proteins/antagonists & inhibitors/*genetics/metabolism
;
*Membrane Transport Proteins
;
Microinjections
;
Molecular Sequence Data
;
Nerve Tissue Proteins/antagonists & inhibitors/*genetics/metabolism
;
Oocytes/metabolism
;
*Organic Anion Transporters
;
Poly A/analysis
;
RNA, Messenger/analysis
;
Rats
;
Sodium/physiology
;
Structure-Activity Relationship
;
Xenopus
;
gamma-Aminobutyric Acid/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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