Publication Date:
2007-04-07
Description:
Inositol pyrophosphates are a diverse group of high-energy signaling molecules whose cellular roles remain an active area of study. We report a previously uncharacterized class of inositol pyrophosphate synthase and find it is identical to yeast Vip1 and Asp1 proteins, regulators of actin-related protein-2/3 (ARP 2/3) complexes. Vip1 and Asp1 acted as enzymes that encode inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase activities. Alterations in kinase activity led to defects in cell growth, morphology, and interactions with ARP complex members. The functionality of Asp1 and Vip1 may provide cells with increased signaling capacity through metabolism of IP6.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Mulugu, Sashidhar -- Bai, Wenli -- Fridy, Peter C -- Bastidas, Robert J -- Otto, James C -- Dollins, D Eric -- Haystead, Timothy A -- Ribeiro, Anthony A -- York, John D -- 2-P30-CA14236-3/CA/NCI NIH HHS/ -- P30-CA-14236/CA/NCI NIH HHS/ -- R01-HL-55672/HL/NHLBI NIH HHS/ -- R33-DK-070272/DK/NIDDK NIH HHS/ -- New York, N.Y. -- Science. 2007 Apr 6;316(5821):106-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Howard Hughes Medical Institute, Department of Pharmacology and Cancer Biology, Duke University Medical Center, DUMC 3813, Durham, NC 27710, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17412958" target="_blank"〉PubMed〈/a〉
Keywords:
Actin-Related Protein 3/metabolism
;
Amino Acid Sequence
;
Animals
;
Catalytic Domain
;
Chromatography, High Pressure Liquid
;
Conserved Sequence
;
Cytoskeletal Proteins/chemistry/genetics/isolation & purification/*metabolism
;
Humans
;
Inositol Phosphates/metabolism
;
Molecular Sequence Data
;
Phosphorylation
;
Phosphotransferases (Phosphate Group Acceptor)/chemistry/genetics/isolation &
;
purification/*metabolism
;
Phytic Acid/*metabolism
;
Protein Structure, Tertiary
;
Recombinant Fusion Proteins/chemistry/isolation & purification/metabolism
;
Saccharomyces cerevisiae/*enzymology/genetics
;
Saccharomyces cerevisiae Proteins/chemistry/genetics/isolation &
;
purification/metabolism
;
Schizosaccharomyces/cytology/*enzymology/genetics/growth & development
;
Schizosaccharomyces pombe Proteins/chemistry/genetics/isolation &
;
purification/*metabolism
;
*Sequence Alignment
;
Substrate Specificity
;
Temperature
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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