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  • Chemistry  (101)
  • Inorganic Chemistry  (26)
  • Amino Acid Sequence  (17)
  • Life and Medical Sciences  (12)
  • 1
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    Development, validation, and utilization of a competitive enzyme-linked immunosorbent assay for the detection of antibodies against Brucella species in marine mammals (2021)
    In:  jennymeegan@hotmail.com | http://aquaticcommons.org/id/eprint/14839 | 403 | 2014-02-28 22:55:34 | 14839 | United States National Ocean Service
    Details
    Publication Date: 2021-06-26
    Description: A competitive enzyme-linked immunosorbent assay (cELISA) was developed by using a whole-cell antigen from a marine Brucella sp. isolated from a harbor seal (Phoca vitulina). The assay was designed to screen sera from multiple marine mammal species for the presence of antibodies against marine-origin Brucella. Based on comparisons with culture-confirmed cases, specificity and sensitivity for cetacean samples tested were 73% and 100%, respectively. For pinniped samples, specificity and sensitivity values were 77% and 67%, respectively. Hawaiian monk seal (Monachus schauinslandi; n = 28) and bottlenose dolphin (Tursiops truncatus; n = 48) serum samples were tested, and the results were compared with several other assays designed to detect Brucella abortus antibodies. The comparison testing revealed the marine-origin cELISA to be more sensitive than the B. abortus tests by the detection of additional positive serum samples. The newly developed cELISA is an effective serologic method for detection of the presence of antibodies against marine-origin Brucella sp. in marine mammals.
    Keywords: Chemistry ; Fisheries ; Management
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 856-862
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  • 2
    Electronic Resource
    Electronic Resource
    Photooxidation of polystyrene by O2 and N2O (1992)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part A: Polymer Chemistry 30 (1992), S. 2677-2681 
    Details
    ISSN: 0887-624X
    Keywords: polystyrene ; photooxidation ; oxygen ; nitrous oxide ; ultraviolet ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Strips of polystyrene held in a flowing O2 or N2O atmosphere have been exposed to 240-600 nm radiation. The extent of photooxidation has been followed by x-ray photoelectron spectroscopy (XPS). Although N2O is a more reactive gas than O2, it produces a less oxidized polymer surface. This surprising observation can be correlated to the photochemistry occurring at the gas/polystyrene interface. © 1992 John Wiley & Sons, Inc.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pola.1992.080301302
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  • 3
    Electronic Resource
    Electronic Resource
    On-column injection technique for use with 100 μm I.D. capillary columns (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 697-699 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography, GC ; Capillary columns, 100 μm I.D. ; On-column injector ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240071206
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  • 4
    Electronic Resource
    Electronic Resource
    Interlaboratory studies of the determination of selected chlorobiphenyl congeners with capillary gas chromatography using splitless- and on-column injection techniques (1985)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 8 (1985), S. 475-480 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatrography, GC ; Capillary columns ; Splitless and on-column injection ; Interlaboratory study ; Chlorobiphenyl congeners ; Internal standards ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The Community Bureau of Reference has organized a collaborative interlaboratory project to improve the analytical protocol for some specific chlorobiphenyls (CBs) within the European Community (EC). A series of test procedures were prescribed to optimize the gas chromatographic conditions for splitless and on-column injection, which substantially improved the quality of data. Important parameters included the initial column and injector temperatures and the choice of the polarity of the stationary phase. In a study with cleaned eel-fat extracts, coefficients of variation for reproducibility CV(R) ranging from 11 to 24% at the 0.04-0.3 mg/kg level per CB congener were obtained. No significant difference could be found between splitless- and on-column injection.
    Additional Material: 4 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240080824
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  • 5
    Electronic Resource
    Electronic Resource
    Evaluation of electron capture cell designs for use in high resolution capillary column gas chromatography (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 427-430 
    Details
    ISSN: 0935-6304
    Keywords: Capillary GC ; Electron capture detector ; Cell design ; Time constant, purge flow ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A comparison of the effects on peak shape is made between a 350 and 100 microliter displaced coaxial and a 350 microliter concentric cylinder electron capture cell. Peaks as fast as W1/2 = 0.5 second were detected using electronics operating in the constant current mode. The measured peak shapes are compared to those obtained by computer simulation assuming either complete mixing or plug-like flow within ECD cell. The advantage of controlling the flow pattern through the cell to obtain plug-like flow is demonstrated. Differences in peak shape between the displaced coaxial and concentric cylinder cells of the same volume are explained in terms of the electrostatic fields within the cells.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060805
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  • 6
    Electronic Resource
    Electronic Resource
    A micro-volume electron capture detector for use in high resolution capillary column gas chromatography (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 651-654 
    Details
    ISSN: 0935-6304
    Keywords: Capillary GC ; Electron capture detector ; Cell design ; Hydrogen carrier gas ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A new design for an electron capture detector cell is described. The cell is compatible with the requirements of high resolution capillary columns and is shown to be useful in applications that require high analysis speed. A unique method of sample introduction reduces the problems of sample loss by adsorption on the surfaces of the cell. Previously reported problems of sample loss by adsorption on active surfaces within the cell when using hydrogen carrier gas are shown to have been eliminated. Examples are shown demonstrating the increased speed of analysis that can be obtained when using hydrogen carrier gas and 100 micron diameter columns.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240061203
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  • 7
    Electronic Resource
    Electronic Resource
    A study of photosensitive insecticidal volatile compounds in marigold (Tagetes minuta) (1993)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 16 (1993), S. 53-55 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Plant extract ; Photochemical degradation ; Insecticides ; Volatile compounds ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240160111
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  • 8
    Electronic Resource
    Electronic Resource
    Membrane preconcentration-capillary electrophoresis-mass spectrometry (mPC-CE-MS) analysis of 3-phenylamino-1,2-propanediol (PAP) metabolites (1996)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 19 (1996), S. 291-294 
    Details
    ISSN: 0935-6304
    Keywords: Capillary electrophoresis-mass spectrometry ; Membrane preconcentration ; Capillary electrophoresis ; Sulfonated membrane ; Polar drug metabolites ; Toxic oil syndrome ; Eosinophilia-myalgia syndrome ; 3-Phenylamino-1,2-propanediol ; 3-Phenylamino-L-alanine ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240190511
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  • 9
    Electronic Resource
    Electronic Resource
    Dicholorbenzyl alkyl ether homologs as retention index markers and internal standards for the analysis of environmental samples using capillary gas chromatography (1985)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 8 (1985), S. 443-449 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography, GC ; Fused silica capillary columns ; Retention index ; Internal standards ; Environmental samples ; Dichlorobenzyl alkyl ethers ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A homologous series of 2,4-dichlorobenzyl alkyl ethers (DCBEs) have been synthesized and purified for use as retention index calibrants and internal standards. They are stable, sensitive to ECD and FID and ideal for GC-MS, with base peaks at m/z 159/161. The retention index calibration against the n-alkanes for a linear temperature program (LTP) series is given and recommendations made for the most suitable members of the DCBE series for use as internal standards in organochlorine (OC) residue analysis.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240080818
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  • 10
    Electronic Resource
    Electronic Resource
    Enhanced mass resolution in a quadrupole ion trap (1993)
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 7 (1993), S. 43-45 
    Details
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: The versatility of the quadrupole ion trap as a mass spectrometer in which the mass resolution may be varied over a wide range has been demonstrated. Mass resolution of 0.8 × 107 and 1.2 × 107 have been obtained for ions of m/z 414 and m/z 614, respectively, well in excess of the normal value of 3m, where m is the mass of ion. At the highest mass resolution, peak-widths at half-maximum are but 52 × 10-6 u. While it has been shown previously that enhanced mass reslution in the quadrupole ion trap can be achieved by reduction of the mass scanning rate, the range of mass resolutions reported here were achieved by reducing the mass scanning rate, in stages, by an overall factor of ca 5.5 × 104.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/rcm.1290070111
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