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  • 1
    ISSN: 1432-2048
    Keywords: Ammonia ; Meiosis ; Protein metabolism ; Proteinases ; Saccharomyces ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Meiosis and sporogenesis in yeast are completely blocked by ammonia added in low concentration (10 mM) to the sporulation medium. Premeiotic DNA synthesis is not initiated in the presence of ammonium ions. The inhibitor interferes with protein turnover by reducing both synthesis and breakdown. The in vitro activities of proteinases A and B in sporulation medium supplemented with ammonia are much lower than in the control. This may partially explain the effect of ammonium ions on protein metabolism in vivo.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 102 (1975), S. 117-122 
    ISSN: 1432-072X
    Keywords: Actinomycin ; Allomyces ; Gametogenesis ; Nuclear Cap ; Polyribosomes ; RNA Synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Synchronous gametogenesis in the water mold Allomyces arbuscula is blocked by actinomycin D added at the onset of the process. Formation of the male gametangium can be selectively inhibited by administering actinomycin one hr after the induction of gametogenesis. The polyribosome pattern obtained after density gradient centrifugation remains virtually unchanged throughout gametogenesis until a stage immediately preceding maturation of the gametes. When ribosomes from gametes and swarming zygotes are analyzed on gradients, some RNase-sensitive material is found to band in the heavier portion of the gradient. Its presence suggests that some messenger RNA associated with ribosomes is conserved in the swarming cells. During gametogenesis RNA is de novo synthesized and becomes associated with the polyribosomes.
    Type of Medium: Electronic Resource
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