Publication Date:
2024-05-27
Description:
We assessed the responses of solitary cells of Arctic Phaeocystis pouchetii (Strain PS78) grown under a matrix of temperature (2°C vs. 6°C), light intensity (55 vs. 160 μmol photons m-2 s-1) and CO2 partial pressures (pCO2; 400 vs. 1000 μatm). Before the experiments, the strain (isolated during Polarstern cruise PS78 in 2011) was kept as stock culture at 1° in 0.2 µm sterile-filtered Arctic seawater (Salinity 33), enriched with vitamins and trace metals according to F/2 medium (Guillard & Ryther, 1962). Nitrate and phosphate were added in concentrations of 100 and 6 µmol L-1, respectively. Experiments were conducted between May 2016 and September 2017 at the Alfred-Wegener-Institute, using standardized media and continuous light exposition. Next to acclimation parameters (growth rates, particulate and dissolved organic carbon and nitrogen, chlorophyll a content), we measured physiological processes in-vivo (electron transport rates and net photosynthesis) using fast-repetition rate fluorometry and membrane-inlet mass spectrometry.
Keywords:
Alkalinity, total; Bottle incubation; calculated from carbonate chemistry using the CO2Sys Excel sheet (Pierrot, Lewis & Wallace, 2006); calculated from chlorophyll a (chl a) and particulate organic carbon (POC) quota; calculated from growth rate and particulate organic carbon (POC) quota; calculated from growth rate and particulate organic nitrogen (PON) quota; calculated from particulate organic carbon (POC) and particulate organic nitrogen (PON) quota; Carbon, inorganic, dissolved; Carbon dioxide, partial pressure; Colorimetric detection, TRAACs continuous flow autoanalyzer, according to the method of Stoll et al. (2001); Coulter counter, Beckman Coulter, Multisizer 3; DATE/TIME; Electron transport rate, relative; Elemental analyzer, EuroVector, EuroEA; EXP; Experiment; Experimental treatment; Fitted parameter using the photosynthesis vs. Irradiance equation from Rokitta & Rost (2012), raw data obtained using a membrane-inlet mass spectrometer (MIMS) as described in Kottmeier, Rokitta & Rost (2016); Fitted parameter using the photosynthesis vs. Irradiance equation from Rokitta & Rost (2012); raw data obtained using a fast-repetition rate fluoremeter (FRRF), FastOcean PTX with FastAct Laboratory system, Chelsea Technologies after Oxborough et al. (201; Fluorometer, Turner Designs, TD-700, using acidification method (Knap et al., 1996); Fram Strait; Identification; Initial slope of the photosynthesis-irradiance curve; Initial slope of the photosynthesis-irradiance curve, relative electron transfer rate per unit light; Light; Light acclimation index; Maximum photosynthesis rate, oxygen, per chlorophyll a; model simulation; pCO2; pCO2 mixed from CO2-free air and pure CO2 with a custom built gas mixing system; pH; pH 826 mobile handheld device, with Aquatrode Plus, Metrohm; Phaeocystis_pouchetii_PS78; Phaeocystis pouchetii; Phaeocystis pouchetii, carbon, organic, particulate/nitrogen, organic, particulate ratio; Phaeocystis pouchetii, chlorophyll a/carbon, organic, particulate ratio; Phaeocystis pouchetii, chlorophyll a quota per cell; Phaeocystis pouchetii, growth rate; Phaeocystis pouchetii, particulate organic carbon production per cell; Phaeocystis pouchetii, particulate organic carbon quota per cell; Phaeocystis pouchetii, particulate organic nitrogen production per cell; Phaeocystis pouchetii, particulate organic nitrogen quota per cell; Phytoplankton; RCP8.5; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Strain; Temperature; Temperature, water; Thermometer, internal, Aquatrode Plus, Metrohm; Treatment: light intensity; Treatment: partial pressure of carbon dioxide; Treatment: temperature; Type of study; Universal light meter & data logger, WALZ, ULM-500, with 4Pi sensor, LI-COR
Type:
Dataset
Format:
text/tab-separated-values, 908 data points
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