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  • Articles  (23)
  • Life and Medical Sciences  (13)
  • Chemistry  (10)
  • Aircraft Propulsion and Power
  • Atomic and molecular processes in external fields, including interactions with strong fields and short pulses
  • Fundamental concepts
  • Philippinen
  • Biology  (16)
  • Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics  (7)
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  • Articles  (23)
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  • 1
    Electronic Resource
    Electronic Resource
    Kinetics of alcohol fermentation at high yeast levels (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 21 (1979), S. 1477-1482 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260210814
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  • 2
    Electronic Resource
    Electronic Resource
    Dielectric relaxation processes in a brick-like metallomesogen ferroelectric liquid crystalThis work was supported by the Spanish Government (projects: CICYT Mat-94-0717-C02-02 and DGCYT PB91-0554) and the European Community (Human Capital and Mobility Programme ERB4050 Pl922749). Santos Merino thanks the Basque Government for provision of his grant. (1996)
    Weinheim : Wiley-Blackwell
    Advanced Materials 8 (1996), S. 644-647 
    Details
    ISSN: 0935-9648
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/adma.19960080808
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  • 3
    Electronic Resource
    Electronic Resource
    Dielectric relaxation processes in an antiferroelectric liquid crystalThis work was supported by the CICYT (Spain, projects MAT-94-0717-C02-02 and Mat-91-0923-C02-02) and the EC Capital and Mobility Programme (ERB4050 PL922749). Santos Merino and Yolanda González thank the Basque Government for a fellowship (1995)
    Weinheim : Wiley-Blackwell
    Advanced Materials 7 (1995), S. 564-568 
    Details
    ISSN: 0935-9648
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/adma.19950070612
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  • 4
    Electronic Resource
    Electronic Resource
    Broadband dielectric spectroscopy on an oxovanadium metallomesogen compoundThis work was supported by the CICYT (Spain, project MAT-91-0962-C02-02) and the EC Human Capital and Mobility Programme (ERB4050 PL922749). (1994)
    Weinheim : Wiley-Blackwell
    Advanced Materials 6 (1994), S. 941-944 
    Details
    ISSN: 0935-9648
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/adma.19940061208
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  • 5
    Electronic Resource
    Electronic Resource
    Use of anti-idiotypic antibodies as probes for the interaction of TSH subunits with its receptor (1987)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 34 (1987), S. 151-162 
    Details
    ISSN: 0730-2312
    Keywords: anti-idiotypic antibodies ; thyrotropin subunits ; thyrotropin receptor ; monoclonal antibody ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: TSH is a heterodimeric glycoprotein hormone, whose dissociated subunits are without biological activity. This has precluded the assessment of the relative contribution of each subunit to hormone action. We have raised anti-idiotypes to monoclonal antibodies specific, respectively, for the α and β hTSH subunits. The anti-β anti-idiotype inhibited l25I-hTSH binding to the β subunit-specific monoclonal quantitatively, whereas 125I-hTSH binding to the α subunit-specific monoclonal was not inhibited by anti-α anti-idiotypes, suggesting that only the former is an “internal image” anti-idiotype. Neither of the two anti-idiotypes nor equimolar mixtures thereof inhibited 125I-bTSH binding to thyroid membranes, even though radiolabelled anti-idiotypes showed saturable binding to thyroid plasma membrane which was inhibited 41-65% by bTSH. Each anti-idiotype alone caused 9% inhibition (compared to 50% by NRIgG) of thyroid plasma membrane adenylate cyclase. Equimolar mixtures (125 μg/ml IgG of each anti-idiotype) induced enzyme activity equivalent to 85% of that of 250 mU/ml of TSH. The TSH-like action of the two anti-idiotypes was also reflected in their capacity to increase (450% by 250 μg/ml IgG compared to normal rabbit IgG) the uptake of 131I into isolated thyrocytes and to promote the organization of such cells into follicular structures. At 250 μg/ml, anti-β anti-idiotype promoted the organization of small follicles and only at a concentration of 500 μg/ml did it enhance 131I uptake. Anti α idiotype was without effect in both assays. Lastly, mixture of anti-idiotypes bound to the ∼ 197,000 Mr band (TSH holoreceptor) on protein blots of thyroid plasma membranes resolved on NaDSO4-polyacrylamide gel electrophoresis under non-reducing conditions. Individual anti-idiotypes were without effect.The TSH α and β subunits apparently deliver two cooperative signals to the receptor and that specificity is associated with the β subunit, while the α subunit is important in enhancing receptor affinity for the heterodimer and in stablizing TSH-receptor complex.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240340302
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  • 6
    Electronic Resource
    Electronic Resource
    Biologic activity of anti-thyrotropin anti-idiotypic antibody (1982)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 19 (1982), S. 305-313 
    Details
    ISSN: 0730-2312
    Keywords: anti-iodiotypic antibody ; thyrotropin ; receptor ; thyroid stimulating antibody ; Graves disease ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: We raised an antihuman thyrotropin anti-idiotypic antibody and showed that it was active at the thyrotropin receptor. Thus this antibody inhibited 125I b-TSH binding to thyroid plasma membranes, stimulated adenylate cyclase activity through a guanyl nucleotide-dependent mechanism, increased radioiodide entry rate into isolated porcine thyroid follicular cells, and induced such cultured cells to organize into follicles. All these parameters are typical of thyrotropin action. This work raises the possibility that thyroid stimulating antibodies that cause the hyperthyroidism of Graves disease may be, at least in some patients, anti-thyrotropin anti-idiotypic antibodies. It also offers a novel method whereby antireceptor antibodies used in the isolation and characterization of the receptor may be raised from ligands.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240190402
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  • 7
    Electronic Resource
    Electronic Resource
    Immobilization of luciferase from a firefly lantern extract on glass strips as an alternative strategy for luminescent detection of ATP (1998)
    New York : Wiley-Blackwell
    Journal of Bioluminescence and Chemiluminescence 13 (1998), S. 371-378 
    Details
    ISSN: 0884-3996
    Keywords: bioluminescence ; luciferase ; ATP ; immobilization ; glass ; poly-L-lysine ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: The bioluminescent reaction catalysed by firefly luciferase has become widely established as an outstanding analytical system for assay of ATP. When used in solution, luciferase is unstable and cannot be re-used, a problem that can be partially circumvented by immobilizing the enzyme on solid substrates. Transparent glass is especially advantageous over alternative immobilizing matrices, since it allows most of the emitted photons to be detected. We report a new method for luciferase immobilization on glass which does not require prior silanization and glutaraldehyde activation, thus saving preparation time and minimizing enzyme inactivation. Our method is based on the co-immobilization by adsorption of luciferase (from a firefly lantern extract) and poly-L-lysine (PL) on non-porous glass strips. Luciferase immobilized in this way exhibits minimal variations in intersample activity, high sensitivity for ATP detection (linear luminescence responses down to 50 nmol/L) and good stability (full activity for at least 60 days when stored at -80°C). PL-mediated immobilization of luciferase on glass strips provides an attractive strategy for the design of specific ATP biosensors, with potential in industry, environmental screening, medicine and biological research. © 1998 John Wiley & Sons, Ltd.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Activation of the C-fos promoter by increased internal pH (1995)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 57 (1995), S. 630-640 
    Details
    ISSN: 0730-2312
    Keywords: internal pH ; transformation ; c-fos ; AP-1 ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Changes in intracellular pH (pHin) take part in the mitogenic response. Their importance has been stressed by the finding that mouse fibroblasts expressing a yeast proton pumping ATPase (PMA1) exhibit a transformed phenotype and are tumorigenic. These cells do maintain a higher pHin, supporting the idea that elevated pHin may act as a proliferative trigger. Here we show that cells constitutively expressing PMA1 have higher levels of the AP-1 transcription factor. The use of stable transfectants and transient transfection assays show that PMA1 activity induces transactivation of the c-fos promoter. The activation of the promoter is mediated throughout the serum response element (SRE). The use of protein kinase C inhibitors suggests that AP-1 activation is achieved through a pathway independent of protein kinase C.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240570407
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  • 9
    Electronic Resource
    Electronic Resource
    Targeting of aequorin for calcium monitoring in intracellular compartments (1994)
    New York : Wiley-Blackwell
    Journal of Bioluminescence and Chemiluminescence 9 (1994), S. 177-184 
    Details
    ISSN: 0884-3996
    Keywords: Photoproteins ; calcium ; organelles ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: We have recently developed a new method for monitoring Ca2+ concentrations in defined cell compartments. The cDNA encoding the Ca2+-sensitive photoprotein aequorin has been modified in order to include specific targeting sequences and expressed in eukaryotic cells; the recombinant protein, specifically located inside the cells, has allowed the direct study of mitochondrial and nuclear Ca2+ concentrations in living cells. The principles, and the application, of this new methodology are discussed in this article.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bio.1170090312
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  • 10
    Electronic Resource
    Electronic Resource
    Crystallization kinetics of a PEEK/LCP blend (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 55 (1995), S. 233-246 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The crystallization kinetics of a polyetheretherketone (PEEK)/liquid crystalline polymer (LCP) blend was studied by using differential scanning calorimetry. Nonisothermal runnings were performed on heating and on cooling at different rates. Isothermal crystallization experiments at 315, 312, 310, and 307°C, from the melt state (380°C) were performed in order to calculate the Avrami parameters n and k and the fold surface free energy, σe. Polarized light optical micrographs were also obtained to confirm the Avrami predictions. It was observed that the LCP retarded the PEEK crystallization process and that the PEEK melting temperature decreased with the amount of LCP, but the LCP melting temperature increased with the amount of PEEK. Probably the PEEK improves the perfection of the LCP crystalline domains. A spherulitic morphology in pure PEEK and its blends was predicted by the Avrami analysis; however this morphology was only observed for pure PEEK and for the 80/20 composition. The other compositions presented a droplet and fibrillar-like morphology. The overall crystallization rate was observed to decrease with the crystallization temperature for all compositions. Finally, σe was found to decrease with the increase of LCP in the blends, having unrealistic negative values. Thus, calculations were made assuming σe constant at all compositions. It was observed that δ, the interfacial lateral free energy, decreased but still remained positive. It was concluded that in these blends neither σe nor σ could be considered constant. © 1995 John Wiley & Sons, Inc.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1995.070550206
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