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  • Alnus glutinosa  (2)
  • Agroinfection, virulence genes  (1)
  • Daucus (somatic embryogenesis)  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Nitrogen metabolism in actinorhizal nodules of Alnus glutinosa: expression of glutamine synthetase and acetylornithine transaminase (1996)
    Springer
    Plant molecular biology 32 (1996), S. 1177-1184 
    Details
    ISSN: 1573-5028
    Keywords: actinorhiza ; nodules ; Alnus glutinosa ; symbiotic nitrogen fixation ; nitrogen metabolism ; glutamine synthetase ; acetylornithine transaminase ; in situ hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two nodule cDNA clones representing genes involved in Alnus glutinosa nitrogen metabolism were analysed. ag11 encoded glutamine synthetase (GS), the enzyme responsible for ammonium assimilation, while ag118 encoded acetylornithine transaminase (AOTA), an enzyme involved in the biosynthesis of citrulline, the nitrogen transport form in Alnus. GS mRNA was found at highest levels in root nodules, where it was present in the infected cells as well as in the cells of the pericycle of the vascular system. AOTA transcripts were found at high levels in nodules, confined to the infected cells, suggesting that in nodules of A. glutinosa, citrulline biosynthesis takes place mainly in the infected cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00041403
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Tunicamycin-inhibited carrot somatic embryogenesis can be restored by secreted cationic peroxidase isoenzymes (1991)
    Springer
    Planta 184 (1991), S. 478-486 
    Details
    ISSN: 1432-2048
    Keywords: Daucus (somatic embryogenesis) ; Embryogenesis (somatic) ; Peroxidase, cationic (isoenzymes) ; Protein glycosylation ; Glycoprotein secretion ; Somatic embryogenesis ; Tunicamycin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Somatic embryogenesis of carrot (Daucus carota L.) is inhibited by the glycosylation inhibitor tunicamycin. This inhibition is reversible by the addition of correctly glycosylated glycoproteins which have been secreted into the culture medium. To identify the proteins responsible for complementation, glycoproteins present in the medium of embryo cultures were purified and tested for their activity in the tunicamycin inhibition/ complementation assay. A 38-kDa glycoprotein was purified that could restore embryogenesis to more than 50% of that in untreated controls. This 38-kDa glycoprotein was identified as a heme-containing peroxidase on the basis of its A405/A280 ratio (Reinheit Zahl or RZ) and enzyme activity. The 38-kDa peroxidase consisted of four different cationic isoenzymes of which only one or possibly two appeared active in the complementation assay. The cationic peroxidase isoenzymes from the carrot medium could be effectively replaced by cationic horseradish peroxidases which depended on their catalytic properties for their ability to restore tunicamycin-inhibited somatic embryogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00197895
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  • 3
    Electronic Resource
    Electronic Resource
    Detection of T-DNA transfer to plant cells by A. tumefaciens virulence mutants using agroinfection (1986)
    Springer
    Molecular genetics and genomics 205 (1986), S. 411-416 
    Details
    ISSN: 1617-4623
    Keywords: A. tumefaciens ; Agroinfection, virulence genes ; T-DNA transfer ; Cauliflower mosaic virus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary To test whether virulence mutants of Agrobacterium tumefaciens are capable of promoting T-DNA transfer into plant cells, a tandem array of Cauliflower Mosaic Virus (CaMV) DNA was cloned between T-region border sequences on a wide host range plasmid and introduced into various virulence mutants. The resulting strains were used to infect Brassica rapa cv. Just Right. This assay, recently referred to as agroinfection, is based on the appearance of viral symptoms following transfer of T-DNA to plant cells, and is shown to be at least 100 times more sensitive in detecting T-DNA transfer than tumour formation. Mutants in the loci vir A, B and G, which were avirulent on turnip, failed to induce virus symptoms. Of the two vir D mutants tested, neither induced tumours, but one was capable of inducing virus symptoms. Mutants in vir E, C and F, which induced respectively no, small and normal tumours on turnip, all induced virus symptoms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00338075
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  • 4
    Electronic Resource
    Electronic Resource
    Expression of Frankia nif genes in nodules of Alnus glutinosa (1995)
    Springer
    Plant and soil 170 (1995), S. 371-376 
    Details
    ISSN: 1573-5036
    Keywords: actinorhiza ; Alnus glutinosa ; Frankia ; in situ hybridization ; nifH ; nifW ; nifZ
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Expression of Frankia genes involved in nitrogen fixation was studied in Alnus glutinosa nodules using the in situ hybridization technique. The results show that high level expression of nif genes does not occur immediately upon infection of cortical cells by Frankia. Also, only in the infected cells near the tips of the nodule lobes, nif genes are expressed at high levels. In the majority of infected cells, nif gene expression is rather low.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00010491
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