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  • Agrobacterium tumefaciens  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Regeneration of flax plants transformed by Agrobacterium rhizogenes (1988)
    Springer
    Plant molecular biology 11 (1988), S. 551-559 
    Details
    ISSN: 1573-5028
    Keywords: Agrobacterium rhizogenes ; Agrobacterium tumefaciens ; Linum usitatissimum ; plant regeneration ; Ri plasmid ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Regeneration of flax (Linum usitatissimum) following transformation by either Agrobacterium tumefaciens carrying a disarmed Ti-plasmid vector, or Agrobacterium rhizogenes carrying an unmodified Ri plasmid, was examined. Hypocotyl and cotyledon explants inoculated with A. tumefaciens formed transformed callus, but did not regenerate transformed shoots either directly or via callus. However, cotyledon explants inoculated with A. rhizogenes formed transformed roots which did regenerate transformed shoots. Ri T-DNA encoded opines were detected in the transformed plantlets and Southern hybridization analysis confirmed the presence of T-DNA from the Ri plasmid in their DNA. Transformed plantlets had curled leaves, short internodes and some had a more developed root system characterized by plagiotropic behaviour.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00017455
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  • 2
    Electronic Resource
    Electronic Resource
    The pTiC58 tzs gene promotes high-efficiency root induction by agropine strain 1855 of Agrobacterium rhizogenes (1990)
    Springer
    Plant molecular biology 14 (1990), S. 785-792 
    Details
    ISSN: 1573-5028
    Keywords: Agrobacterium rhizogenes ; Agrobacterium tumefaciens ; root induction ; tzs ; Linum usitatissimum L. ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Root induction on flax (Linum usitatissimum L.) cotyledon explants by Agrobacterium rhizogenes strain 1855 is markedly increased by co-inoculation with disarmed A. tumefaciens strain LBA 4404 containing a plasmid carrying the tzs gene of pTiC58. Most of the roots (estimated to be more than 90%) were transformed. This effect is most likely due to the secretion of trans-zeatin by A. tumefaciens stimulating the division of plant cells making them more receptive to transformation by A. rhizogenes, although other explanations are possible. This observation supports the idea that the tzs gene, although not essential for transformation, may promote transformation. An obvious application for genetic engineering experiments involving transformation by A. rhizogenes, is to include a vir-induced tzs gene in the transformation system to help maximize transformation efficiency.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00016511
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    Paper (German National Licenses)
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