ISSN:
1573-9368
Keywords:
Peanut
;
Arachis hypogaea
;
transformation
;
callus
;
Agrobacterium tumefaciens
;
peanut stripe virus coat protein
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Transformed callus was produced from peanut (Arachis hypogaea L. cv. Okrun) hypocotyl explants after four days of co-cultivation withAgrobacterium tumefaciens strains EHA101, LBA4404 or ASE1 carrying the binary vector pKYLX71GUS on a defined medium followed by selection with kanamycin (200 mg l−1). Transformed calluses were cultured as independent cell lines potentially derived from a single transformation event. Stable integration and expression of foreign gene(s) in the callus was confirmed by Southern and western blot analyses and enzyme assays. A few cell lines showed a single insert of the foreign gene. Using the above protocol, transformed peanut callus expressing the peanut stripe virus coat protein gene was obtained.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01976172
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