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  • 1
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    Berlin, Heidelberg : Springer
    Keywords: Biochemistry ; Chemistry, Organic ; Nanotechnology ; Polymers
    ISBN: 9783540314745
    Language: English
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  • 2
    ISSN: 1617-4623
    Keywords: Agrobacterium tumefaciens ; fixNOQP operon ; respiration ; cytochromec oxidase ; Rhizobiaceae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hybridization analysis using theRhizobium meliloti nitrogen fixation genefixN as a probe revealed the presence of a homologous DNA region in the phytopathogenic bacteriumAgrobacterium tumefaciens. Hybridization signals were also detected with total DNAs ofRhizobium leguminosarum bv.phaseoli, Rhodobacter capsulatus andEscherichia coli, but not those ofXanthomonas campestris pv.campestris andPseudomonas putida. The hybridizing fragment fromA. tumefaciens was cloned and sequenced. The predicted gene product of one of the two open reading frames identified on the sequenced fragment shows homology to FixN of differentRhizobiaceae as well as a low but significant similarity to subunit I of heme copper oxidases from various bacteria. The presence of five strictly conserved histidine residues previously implicated in forming ligands to heme and CuB in oxidases and the predicted membrane topology provide evidence that theA. tumefaciens fixN-like gene product is a component of the heme copper oxidase superfamily. The incomplete open reading frame starting only 8 nucleotides downstream of thefixN-like gene exhibits homology toRhizobium fixO. Using anuidA (GUS) gene fusion it could be shown that theA. tumefaciens fixN-like gene is preferentially expressed under microaerobic conditions. Expression of theuidA fusion is abolished inR. meliloti fixJ andfixK mutants, indicating that an Fnr-like protein is involved in transcriptional regulation of thefixN-like gene in A.tumefaciens. The presence of an upstream DNA sequence motif identical to the Fnr-consensus binding site (anaerobox) further supports this hypothesis.A. tumefaciens mutated in thefixN-like gene shows decreased TMPD-specific oxidase activity under microaerobic conditions, indicating that thefixN-like gene or operon codes for proteins involved in respiration under reduced oxygen availability.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 1999 (1999), S. 451-458 
    ISSN: 1434-193X
    Keywords: Cross-coupling ; Modular chemistry ; Repetitive synthesis ; Macrocycles ; Shape persistence ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: -The synthesis of a variety of kinked oligophenylene building blocks is described. The synthetic strategy is repetitive, utilizes the Suzuki cross-coupling protocol, and involves potentially bifunctional building blocks (modules) which carry one functional group for coupling and one group which serves as place holder. After the coupling, the placeholder is converted into a coupling functionality for the next growth step. The building blocks also carry chloro-substituents which are unaffected by all transformations. For one of the large building blocks it is shown that it actually can be cyclized into an oligophenylene macrocycle with a chloro-substituent at each corner and a pair of hexyl chains at each side.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 1998 (1998), S. 99-105 
    ISSN: 1434-193X
    Keywords: Ladder polymers ; Macrocycles ; Cycloadditions ; Retro Diels-Alder reaction ; Benzo[c]furan ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The synthesis of the AB-type Diels-Alder (DA) monomer 12 is reported whose self-condensation leads to the formation of the linear ladder oligomer 17 and its cyclic congener 18. 18 is the largest [n]cyclacene derivative known (n = 18) and can be obtained by thermal treatment of 17 in yields of up to 45% utilizing the reversibility of the DA cyclization involved. 17 can be considered a “storage form” for 18.
    Type of Medium: Electronic Resource
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