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  • Agrobacterium  (1)
  • Key words: Adaptin — Clathrin — Endomembrane system — Eukaryotic evolution — Gene duplication — Multigene family — Phylogeny — Vesicular transport  (1)
  • Mutant (Nicotiana)  (1)
  • Nicotiana (nitrate reductase)  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Phylogenetic Analysis of Components of the Eukaryotic Vesicle Transport System Reveals a Common Origin of Adaptor Protein Complexes 1, 2, and 3 and the F Subcomplex of the Coatomer COPI (1999)
    Springer
    Journal of molecular evolution 48 (1999), S. 770-778 
    Details
    ISSN: 1432-1432
    Keywords: Key words: Adaptin — Clathrin — Endomembrane system — Eukaryotic evolution — Gene duplication — Multigene family — Phylogeny — Vesicular transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Eukaryotic vesicular transport requires the recognition of membranes through specific protein complexes. The heterotetrameric adaptor protein complexes 1, 2, and 3 (AP1/2/3) are composed of two large, one small, and one medium adaptin subunit. We isolated and characterized the cDNA for Arabidopsisγ-adaptin and performed a phylogenetic analysis of all adaptin subunits (proteins) in the context of all known homologous proteins. This analysis revealed (i) that the large subunits of AP1/2/3 are homologous and (ii) two subunits of the heptameric coatomer I (COPI) complex belong to this gene family. In addition, all small subunits and the aminoterminal domain of the medium subunits of the heterotetramers are homologous to each other; this also holds for two corresponding subunits of the COPI complex. AP1/2/3 and a substructure (heterotetrameric, F-COPI subcomplex) of the heptameric COPI had a common ancestral complex (called pre-F-COPI). Since all large and all small/medium subunits share sequence similarity, the ancestor of this complex is inferred to have been a heterodimer composed of one large and one small subunit. The situation encountered today is the result of successive rounds of coordinated gene duplications of both the large and the small/medium subunits, with F-COPI being the first that separated from the ancestral pre-F-COPI.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00006521
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  • 2
    Electronic Resource
    Electronic Resource
    Repair in vitro of nitrate reductase-deficient tobacco mutants (cnxA) by molybdate and by molybdenum cofactor (1985)
    Springer
    Planta 163 (1985), S. 370-375 
    Details
    ISSN: 1432-2048
    Keywords: Molybdenum ; Mutant (Nicotiana) ; Nicotiana (nitrate reductase) ; Nitrate reductase mutant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two nitrate reductase-deficient mutant cell lines (CnxA68/2, CnxA101) of Nicotiana tabacum are shown to be repairable under in-vitro conditions by (i) molybdate or (ii) by preparations of active molybdenum cofactor of homologous or heterologous origin, thereby yielding about 20% and 80%, respectively, of the corresponding wild-type NADH-nitrate reductase (EC 1.6.6.1) activity. In-vitro repair of nitrate reductase activity is dependent on sulphydryl-group protecting reagents and ethylenediaminetetraacetic acid (EDTA) in the extraction medium, the nitrogen source in the growth medium and the age of the cells. The results support the conclusion that the cnxA gene controls the insertion of molybdenum into the molybdenum cofactor. They are consistent with the idea of two interlinked pathways for the metabolic processing of molybdenum acquisition, one involving the synthesis of the structural moiety of the molybdenum cofactor and the other involving processing of the molybdate anion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00395145
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  • 3
    Electronic Resource
    Electronic Resource
    High meiotic stability of a foreign gene introduced into tobacco by Agrobacterium-mediated transformation (1987)
    Springer
    Molecular genetics and genomics 207 (1987), S. 171-175 
    Details
    ISSN: 1617-4623
    Keywords: Nicotiana tabacum ; Agrobacterium ; Plant transformation ; Genetic stability ; Kanamycin resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two lines of transgenic Nicotiana tabacum transformed to kanamycin resistance by means of a binary Agrobacterium vector containing a nos-npt gene were investigated over three generations. Southern hybridization and crossing analyses revealed that a single copy of T-DNA had integrated in each line and that the kanamycin resistance was regularly transmitted to the progeny as a monogenic dominant trait. Homozygous transgenic plants were fully fertile, morphologically normal and did not significantly differ from wild-type plants in the quantitative characters examined (plant height, flowering time). The two lines showed very low, but significantly different levels of meiotic instability: kanamycin-sensitive plants occurred among backcross progeny from homozygous transgenic plants with frequencies of 6/45,000 and 25/45,000, respectively. The sensitive plants arose independently of each other and thus resulted from meiotic rather than mitotic events. These findings demonstrate for the first time that integrated foreign genes can be transmitted to progeny with the high degree of meiotic stability required for commercial varieties of crop plants. They emphasize the importance of non-homologous integration and of avoiding co-integration of inactive gene copies for achieving meiotically stable transformants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331506
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